Connexin 43/GJA1 Antibody - Azide Free

Western Blot: Connexin 43/GJA1 Antibody [NB300-309] - Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.

• Reactivity: Rt, Av, Hu, Mu, Ca, Ch, Pm, Sh, Xp, Ze
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Connexin 43/GJA1 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Connexin 43/GJA1 Antibody - Azide Free (NB300-309) is a polyclonal antibody validated for use in IHC and WB. Anti-Connexin 43/GJA1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P08050
• Marker: Gap Junctions Marker
• Specificity: Specific for endogenous levels of the ~43 kDa connexin43 protein.
• Predicted Species: Human (100%), Mouse (100%), Zebrafish (100%), Sheep (100%), Chicken (100%), Canine (100%), Xenopus (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GJA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 1:1000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Connexin 43/GJA1 Overexpression Lysate

Reactivity Notes

It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Connexin 43/GJA1 Antibody - Azide Free

• Connexin 43
• connexin-43
• CX43
• CX43gap junction protein, alpha-like
• DFNB38
• Gap junction 43 kDa heart protein
• gap junction alpha-1 protein
• gap junction protein, alpha 1, 43kDa (connexin 43)
• gap junction protein, alpha 1, 43kDa
• GJA1
• GJAL
• HSS
• ODD
• ODDD
• ODOD
• SDTY3

Background

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy. Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of Connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C. Phosphorylation of Ser368 by PKC induces a conformational change of Connexin43 that results in a decrease in gap junction permeability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Connexin 43/GJA1 Antibody (NB300-309)(1)

Publication using NB300-309

• Sahin K, Akdemir F, Tuzcu M et al. Genistein suppresses spontaneous oviduct tumorigenesis in quail. Nutr Cancer 2009-01-01 [PMID: 20155619] (WB, Avian)

Bioinformatics

• Gene Symbol: GJA1
• Entrez:
  • 2697(Human)
  • 14609(Mouse)
  • 24392(Rat)
• Uniprot:
  • P08050(Rat)