| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | IHC, IHC-P |
| Clone | 105 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | DyLight 550 |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Connexin 43/GJA1. |
| Predicted Species | Mouse (100%), Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GJA1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-89209AF350 | |
| Alexa Fluor 405 | NBP2-89209AF405 | |
| Alexa Fluor 488 | NBP2-89209AF488 | |
| Alexa Fluor 532 | NBP2-89209AF532 | |
| Alexa Fluor 594 | NBP2-89209AF594 | |
| Alexa Fluor 647 | NBP2-89209AF647 | |
| Alexa Fluor 700 | NBP2-89209AF700 | |
| Alexa Fluor 750 | NBP2-89209AF750 | |
| Biotin | NBP2-89209B | |
| DyLight 350 | NBP2-89209UV | |
| DyLight 405 | NBP2-89209V | |
| DyLight 488 | NBP2-89209G | |
| DyLight 550 | NBP2-89209R | |
| DyLight 594 | NBP2-89209DL594 | |
| DyLight 650 | NBP2-89209C | |
| DyLight 680 | NBP2-89209FR | |
| DyLight 755 | NBP2-89209IR | |
| FITC | NBP2-89209F | |
| HRP | NBP2-89209H | |
| Janelia Fluor 549 | NBP2-89209JF549 | |
| Janelia Fluor 646 | NBP2-89209JF646 |
Diseases for Connexin 43/GJA1 Antibody (NBP2-89209R)Discover more about diseases related to Connexin 43/GJA1 Antibody (NBP2-89209R).
| Pathways for Connexin 43/GJA1 Antibody (NBP2-89209R)View related products by pathway.
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Connexin: Bridging the Gap of Intercellular Communication Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a... Read full blog post. |
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| Gene Symbol | GJA1 |
![Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/images2/Connexin-40-GJA5-Antibody-Immunohistochemistry-Paraffin-NBP1-46568-img0003.jpg)
![Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/images2/Connexin-40-GJA5-Antibody-Immunohistochemistry-Paraffin-NBP1-46568-img0001.jpg)
![Western Blot: Connexin 32/GJB1 Antibody (M12.13) [NBP2-53381] - Western Blot Analysis of human stomach lysate using Connexin 32/GJB1 Antibody (M12.13).](http://images.novusbio.com/images2/Connexin-32-GJB1-Antibody-(M12.13)-Western-Blot-NBP2-53381-img0001.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Western blotting analysis detects Cx26 using R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. (a) R(alpha)Cx26-Ct (A) and R(alpha)Cx26-cl (B) antibodies detected a specific 26kDa band on pig heart tissue lysates (lanes A and B are cropped blots obtained from the same gel). R(alpha)Cx26-cl recognized the 26kDa band on human and rat heart (H) lysates and on rat liver (L) sample (positive control) although two unspecific bands at molecular weights lower than 26kDa were detected. No bands were detected on smooth muscle (SM) tissue lysates (negative control) by R(alpha)Cx26-cl. R(alpha)GAPDH was used as protein loading control for rat tissue samples run on the same gel. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-41304-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Connexin 26/GJB2 Antibody [NBP2-41304] - Cx26 distribution at level of the cardiomyocyte cytoplasm. Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R(alpha)Cx26-cl and M(alpha)Cx43. Scale bar: 20um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Connexin-26-GJB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41304-img0002.jpg)
![Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Human and rat cardiomyocytes immunoreact with R(alpha)Cx26 antibodies. Representative images of immunohistochemical analysis for Cx26 by R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. Longitudinal (c and d) sections showing positivity in cardiomyocytes and in smooth muscle cells of vessel (arrow); Rat (d) immunoperoxidase shows brown immunoreaction in the cell cytoplasm of working cardiomyocytes and in smooth muscle cells of vessels (arrows); positivity of cardiomyocytes and smooth muscle cells has shown, at higher magnification, in longitudinal (d) and cross sections (c); Negative controls are shown in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0004.jpg)
![Western Blot: Connexin 45/GJC1 Antibody [NBP2-76925] - Western blot analysis of Connexin 45 / GJA7 / Cx45 on SH-SY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Lane 1: Anti-Connexin-45 Antibody;Lane 2: Anti-Connexin-45 Antibody, pre-incubated with the immunizaiton peptide.](http://images.novusbio.com/images2/Connexin-45-GJC1-Antibody-Western-Blot-NBP2-76925-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Connexin 45/GJC1 Antibody [NBP2-76925] - ICC staining Connexin 45 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Connexin 45 antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/images2/Connexin-45-GJC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76925-img0003.jpg)
![Immunohistochemistry: Connexin 45/GJC1 Antibody [NBP2-76925] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/images2/Connexin-45-GJC1-Antibody-Immunohistochemistry-NBP2-76925-img0007.jpg)
![Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/Tight-Junction-Protein-1-Antibody-Knockout-Validated-NBP1-85047-img0019.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in human cell line A-431.](http://images.novusbio.com/images2/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Rat epithelial cells stained positively with Tight Junction Protein 1. Primary antibody at 1:100. Secondary antibody: goat anti-rabbit-Alexa 488 conjugated at 1:500. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141] - Staining of MCF7 cells using the Anti-GJB6 monoclonal antibody, showing no specific staining of cell junctions. MCF7 cells serves as a negative control based on RNA-seq values. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/images2/Connexin-30-GJB6-Antibody-(CL4540)-Immunocytochemistry-Immunofluorescence-NBP2-61141-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Connexin 30/GJB6 Antibody (CL4540) [NBP2-61141] - Staining of RT-4 cells using the Anti-GJB6 monoclonal antibody, showing specific staining of cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/images2/Connexin-30-GJB6-Antibody-(CL4540)-Immunocytochemistry-Immunofluorescence-NBP2-61141-img0001.jpg)
![Western Blot: PRRT2 Antibody [NBP2-82026] - Analysis of PRRT2 in mouse brain tissue lysate with PRRT2 antibody at 1 ug/ml.](http://images.novusbio.com/images2/PRRT2-Antibody-Western-Blot-NBP2-82026-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PRRT2 Antibody [NBP2-82026] - Immunofluorescence of PRRT2 in rat brain tissue with PRRT2 antibody at 20 ug/ml.](http://images.novusbio.com/images2/PRRT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82026-img0003.jpg)
![Immunohistochemistry: PRRT2 Antibody [NBP2-82026] - Immunohistochemistry of PRRT2 in rat brain tissue with PRRT2 antibody at 2.5 ug/ml.](http://images.novusbio.com/images2/PRRT2-Antibody-Immunohistochemistry-NBP2-82026-img0002.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/images2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: Jurkat cell lysate GJD2 is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/images2/Connexin-36-GJD2-Antibody-Western-Blot-NBP1-59254-img0006.jpg)
![Immunohistochemistry-Frozen: Connexin 36/GJD2 Antibody [NBP1-59254] - IHC detection of Connexin 36/GJA9 in 50uM thick section of Mouse Brain. The tissues were pre-fixed overnight in 4% PFA, and primary antibody NBP1-59254 was used at a concentration of 1ug/mL (along with Parvalbumin antibody for double staining) with gentle shaking and overnight incubation at 4C. For the detection of NBP1-59254 primary, anti-rabbit Alexa 555 secondary was employed and images were captured using a confocal microscope at 63X resolution.](http://images.novusbio.com/images2/Connexin 36-GJD2 Antibody-Immunohistochemistry-Frozen-NBP1-59254-img0007.jpg)
![Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/images2/Connexin-36-GJD2-Antibody-Western-Blot-NBP1-59254-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.](http://images.novusbio.com/images2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).](http://images.novusbio.com/images/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/GFAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-141-img0003.jpg)
![Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (http://www.translational-medicine.com/content/13/1/227), licensed under a CC-BY licence.](http://images.novusbio.com/images2/N-Cadherin-Antibody-(13A9)-Immunocytochemistry-NBP1-48309-img0025.jpg)
![Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.](http://images.novusbio.com/images2/N-Cadherin-Antibody-(13A9)-Western-Blot-NBP1-48309-img0005.jpg)
![Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Human meningioma mouse xenograft model KCI-MENG1-LPSX generated with the spontaneously immortal cell line KCI-MENG1-LP. Tumors from immunocompromised SCID mice were dissected and the derivative cell line KCI-MENG1-LPSX CL was generated. The EMA, PR, and N-cadherin IHC of the mouse tumor highly resembled the original patient-derived tumor. The vimentin- and Ki-67-stained cells in the mouse tumor tissue were markedly more abundant and more intensely stained than in the original tumor. KCI-MENG1-LPSX CL cells displayed the same patterns of immunostaining as the high passage parent cell line KCI-MENG1-HP, including the loss of PR staining. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (http://www.translational-medicine.com/content/13/1/227), licensed under a CC-BY licence.](http://images.novusbio.com/images2/N-Cadherin-Antibody-(13A9)-Immunocytochemistry-NBP1-48309-img0026.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Western-Blot-NBP2-37576-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Western Blot: Connexin 46/GJA3 Antibody [NBP1-59197] - Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.](http://images.novusbio.com/images2/Connexin-46-GJA3-Antibody-Western-Blot-NBP1-59197-img0003.jpg)