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Rat Complement Factor H ELISA Kit (Colorimetric)

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ELISA: Rat Complement Factor H ELISA Kit (Colorimetric) [NBP3-18771] - Samples were spiked with high concentrations of Rat Complement Factor H and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Rat Complement Factor H ELISA Kit (Colorimetric) [NBP3-18771] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.5-4000 ng/mL
Sensitivity
37.5 ng/mL

Order Details

Rat Complement Factor H ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Complement Factor H in samples. No significant cross-reactivity or interference between Rat Complement Factor H and analogues was observed.
Standard Curve Range
62.5-4000 ng/mL
Sensitivity
37.5 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.54
Intra-Assay
%CV < 5.46
Spike Recovery
86-100%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CFH

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Complement Factor H ELISA Kit (Colorimetric)

  • adrenomedullin binding protein
  • age-related maculopathy susceptibility 1
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • beta-1H
  • beta-1-H-globulin
  • beta-1-H-globulin
  • CFH
  • CFHL3
  • Complement Factor H
  • factor H
  • factor H-like 1
  • FH
  • FHL1
  • H factor 1 (complement)
  • H factor 1
  • H factor 2 (complement)
  • HF
  • HF1
  • HF1ARMS1
  • HF2
  • HUS
  • HUSMGC88246

Background

The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy. Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600mg/L for the 155 kDa form and 1-5mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CFH