Mouse Complement Factor H ELISA Kit (Colorimetric)

ELISA: Mouse Complement Factor H ELISA Kit (Colorimetric) [NBP3-18770] - Samples were spiked with high concentrations of Mouse Complement Factor H and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Complement Factor H ELISA Kit (Colorimetric) [NBP3-18770] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 3.13-200 ng/mL
• Sensitivity: 1.88 ng/mL

Mouse Complement Factor H ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse Complement Factor H in samples. No significant cross-reactivity or interference between Mouse Complement Factor H and analogues was observed.
• Standard Curve Range: 3.13-200 ng/mL
• Sensitivity: 1.88 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.61
• Intra-Assay: %CV < 5.41
• Spike Recovery: 92-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CFH

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Complement Factor H ELISA Kit (Colorimetric)

• adrenomedullin binding protein
• age-related maculopathy susceptibility 1
• AHUS1
• AMBP1
• ARMD4
• ARMS1
• beta-1H
• beta-1-H-globulin
• beta-1-H-globulin
• CFH
• CFHL3
• Complement Factor H
• factor H
• factor H-like 1
• FH
• FHL1
• H factor 1 (complement)
• H factor 1
• H factor 2 (complement)
• HF
• HF1
• HF1ARMS1
• HF2
• HUS
• HUSMGC88246

Background

The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy. Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600mg/L for the 155 kDa form and 1-5mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Complement Factor H ELISA Kit (NBP3-18770). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CFH