Complement Component C9 Antibody (SR2144) - BSA Free

Immunohistochemistry: Complement Component C9 Antibody (SR2144) [NBP3-21635] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21635.

Western Blot: Complement Component C9 Antibody (SR2144) [NBP3-21635] - Western blot analysis using NBP3-21635 in Human fetal liver lysate.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: SR2144
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Complement Component C9 Antibody (SR2144) - BSA Free Summary

• Description: Novus Biologicals Rabbit Complement Component C9 Antibody (SR2144) - BSA Free (NBP3-21635) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Complement Component C9 (Uniprot #: P02748)
• Specificity: Detects endogenous levels of total Complement Component C9
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: C9
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Complement Component C9 Antibody (SR2144) - BSA Free

• complement component 9
• Complement Component C9

Background

C9 is synthesised in the liver and monocytes, and is a plasma protein consisting of a single polypeptide chain of molecular weight 71kDa. Normal plasma concentration is 60mg/L. C9 forms part of the membrane attack complex (MAC) the cytolytic terminal complex of the complement pathways. C9 binds to the membrane associated C5b-8, binding of C9 to C5b-8 leads to the circular polymerisation of 12-18 C9 molecules. This is the basis of the hydrophilic, protein-walled, trans-membrane channel formed by the MAC, which leads to cell lysis and destruction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C9