| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-21612-0.025ml | |||
HAF018). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Complement Component C9 Monoclonal Antibody (Catalog # MAB8126) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8126-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62803-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87440-25ul | |||
HAF016). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human plasma. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Complement Component C9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8126) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Component C9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF8126-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21635-50ul | |||
![<b>Biological Strategies Validation. </b>Western Blot: Complement Component C9 Antibody [NBP2-15952] - Sample (30 ug of whole cell lysate) A: THP-1 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-Western-Blot-NBP2-15952-img0002.jpg)
![Western Blot: Complement Component C9 Antibody [NBP2-15952] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C9 antibody [N2C2-2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-Western-Blot-NBP2-15952-img0005.jpg)
![Western Blot: Complement Component C9 Antibody [NBP2-15952] - Rat plasma (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Complement Component C9 Antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-Western-Blot-NBP2-15952-img0006.jpg)
![Western Blot: Complement Component C9 Antibody [NBP2-15952] - Mouse plasma (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component 9 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody,](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-Western-Blot-NBP2-15952-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis.A generic staining of the vascular BMs was given by an antibody to the 7S domain of collagen IV alpha 3 (A, C, E, F, G). Prominent staining for microvascular aneurisms was detected by staining with antibodies to C9 (B, C), Fibronectin (FN, E), ApoE (F) & PRELP (G). The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins (D). A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0189857), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15952_rabbit-polyclonal-complement-component-c9-antibody-310202416121980.jpg)
![Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis.A generic staining of the vascular BMs was given by an antibody to the 7S domain of collagen IV alpha 3 (A, C, E, F, G). Prominent staining for microvascular aneurisms was detected by staining with antibodies to C9 (B, C), Fibronectin (FN, E), ApoE (F) & PRELP (G). The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins (D). A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0189857), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15952_rabbit-polyclonal-complement-component-c9-antibody-31020241613612.jpg)
![Immunocytochemistry/ Immunofluorescence: Complement Component C9 Antibody [NBP2-15952] - Staining of vascular BM whole mounts with antibodies to proteins detected in the proteome analysis.A generic staining of the vascular BMs was given by an antibody to the 7S domain of collagen IV alpha 3 (A, C, E, F, G). Prominent staining for microvascular aneurisms was detected by staining with antibodies to C9 (B, C), Fibronectin (FN, E), ApoE (F) & PRELP (G). The same treatment of vascular BM whole mounts from non-diabetic eyes did not show staining for these proteins (D). A norrin-specific staining is shown to be generic for the entire vascular BM whole mounts (H), the signal, however, being less prominent in vascular aneurisms (arrow in H). Staining of vascular BM whole mounts from non-diabetic eyes showed a clearly weaker staining for norrin, when compared to vascular whole mounts from non-diabetic donors. Bar: 25um. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0189857), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-15952_rabbit-polyclonal-complement-component-c9-antibody-310202416113518.jpg)
![C9 antibody [N2C2-2], Internal detects C9 protein at by immunofluorescent analysis.</br>Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: C9 stained by C9 antibody [N2C2-2], Internal (NBP2-15952) diluted at 1:500.</br>Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15952_rabbit-polyclonal-complement-component-c9-antibody-immunocytochemistry-immunofluorescence-261120251830388.jpg)
![ELISA: Human Complement Component C9 ELISA Kit (Colorimetric) [NBP2-60560]](http://images.novusbio.com/fullsize/Human-Complement-Component-C9-ELISA-Kit-Colorimetric-ELISA-NBP2-60560-img0001.jpg "ELISA: Human Complement Component C9 ELISA Kit (Colorimetric) [NBP2-60560]")
![Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - Staining of human liver with complement component C9 antibody. Plasma of portal veins are strongly stained.](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-22-BSA-Free-Immunohistochemistry-NBP2-21612-img0004.jpg "Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - Staining of human liver with complement component C9 antibody. Plasma of portal veins are strongly stained.")
![Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - Staining of human kidney with complement component C9 antibody. Plasma of veins are strongly stained.](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-22-BSA-Free-Immunofluorescence-NBP2-21612-img0001.jpg)
![Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - staining of tonsil tissue with complement component C9 antibody. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue.](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-22-BSA-Free-Immunofluorescence-NBP2-21612-img0002.jpg)
![Immunohistochemistry: Complement Component C9 Antibody (22) - BSA Free [NBP2-21612] - staining of tonsil tissue with complement component C9 antibody. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Also moderate staining were observed from plasma of veins.](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-22-BSA-Free-Immunohistochemistry-NBP2-21612-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Complement Component C9 Antibody [NBP1-87440] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Complement-Component-C9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87440-img0008.jpg "Immunocytochemistry/Immunofluorescence: Complement Component C9 Antibody [NBP1-87440] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.")
![Western Blot: Complement Component C9 Antibody (JE01-45) [NBP3-32098] - Western blot analysis of Complement Component C9 on different lysates with Rabbit anti-Complement Component C9 antibody (NBP3-32098) at 1/2,000 dilution.<br/><br/> Lane 1: Human plasma tissue lysate<br/> Lane 2: Human serum tissue lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 63 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 10 seconds ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32098) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32098_rabbit-complement-component-c9-mab-je01-45-27820241631633.jpg "Western Blot: Complement Component C9 Antibody (JE01-45) [NBP3-32098] - Western blot analysis of Complement Component C9 on different lysates with Rabbit anti-Complement Component C9 antibody (NBP3-32098) at 1/2,000 dilution.<br/><br/> Lane 1: Human plasma tissue lysate<br/> Lane 2: Human serum tissue lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 63 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 10 seconds ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32098) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement Component C9 Antibody (JE01-45) [NBP3-32098] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Complement Component C9 antibody (NBP3-32098) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32098) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32098_rabbit-complement-component-c9-mab-je01-45-298202414161841.jpg)
![Immunohistochemistry: Complement Component C9 Antibody (JE01-45) [NBP3-32098] - Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Complement Component C9 with Rabbit anti-Complement Component C9 antibody (NBP3-32098) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32098, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32098_rabbit-complement-component-c9-mab-je01-45-298202414262666.jpg)
![Western Blot: Complement C9 Antibody [NBP2-15951] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Complement-C9-Antibody-Western-Blot-NBP2-15951-img0001.jpg "Western Blot: Complement C9 Antibody [NBP2-15951] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Complement C9 Antibody [NBP2-15951] - Immunohistochemical analysis of paraffin-embedded Normal Colon, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Complement-C9-Antibody-Immunohistochemistry-Paraffin-NBP2-15951-img0002.jpg)
![Immunohistochemistry: Complement Component C9 Antibody (SR2144) [NBP3-21635] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21635.](http://images.novusbio.com/fullsize/nbp3-21635_rabbit-complement-component-c9-mab-sr2144-57202317215125.jpg "Immunohistochemistry: Complement Component C9 Antibody (SR2144) [NBP3-21635] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21635.")
![Western Blot: Complement Component C9 Antibody (SR2144) [NBP3-21635] - Western blot analysis using NBP3-21635 in Human fetal liver lysate.](http://images.novusbio.com/fullsize/nbp3-21635_rabbit-complement-component-c9-mab-sr2144-572023162474.jpg)
![SDS-Page: Recombinant Human Complement Component C9 Protein [H00000735-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Complement-Component-C9-Protein-SDS-Page-H00000735-P01-img0003.jpg "SDS-Page: Recombinant Human Complement Component C9 Protein [H00000735-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
