Complement Component C5a Antibody (295108) [Unconjugated]

Recombinant Mouse Complement Component C5a (Catalog # 2150-C5) inducesN‑acetyl- beta -D-glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N‑Acetyl- beta -D-Glucosaminidase Release elicited by Recombinant Mouse Complement Component C5a (50 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Complement Component C5a Monoclonal Antibody (Catalog # MAB21501). The ND50 is typically 0.1-0.5 µg/mL in the presence of cytochalasin‑B.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clone: 295108
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Complement Component C5a Antibody (295108) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Complement Component C5a
  Asn679-Arg755
  Accession # P06684
• Specificity: Detects mouse Complement Component C5a in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse Complement Component C3d, recombinant human (rh) Complement Component C3a, or rhComplement Component C5a.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Hc
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.1-0.5 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse Complement Component C5a Biotinylated Antibody (Catalog number BAF2150)
  Standard: Recombinant Mouse Complement Component C5a (Catalog number 2150-C5)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C5a Antibody (295108) [Unconjugated]

• C5
• C5A
• Complement Component C5a
• HE

Background

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule (1). Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation (1, 4). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells (1, 4). Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined (5).

1. Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
4. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
5. Okinaga, S. et al. (2003) Biochemistry 42:9406.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Component C5a Antibody (MAB21501)(4)

Publications using MAB21501

• M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells Journal of Neuroinflammation, 2022-07-14;19(1):182. 2022-07-14 [PMID: 35831910] (ICC, Inhibition, Mouse)
• Sun D, Zhang M, Liu G, Wu H, Zhu X, Zhou H, Shi M Real-Time Imaging of Interactions of Neutrophils with Cryptococcus neoformans Demonstrates a Crucial Role of Complement C5a-C5aR Signaling. Infect Immun, 2015-10-26;84(1):216-29. 2015-10-26 [PMID: 26502909] (Neutralization, Mouse)
• Price P, Banki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, Lehmann M Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol, 2014-12-29;194(3):1164-8. 2014-12-29 [PMID: 25548218] (Western Blot, Mouse)
• Kim J, Oh S, Ahn S, Chung D IFN-gamma-producing NKT cells exacerbate sepsis by enhancing C5a generation via IL-10-mediated inhibition of CD55 expression on neutrophils. Eur J Immunol, 2014-05-02;44(7):2025-35. 2014-05-02 [PMID: 24723363] (Mouse)

Bioinformatics

• Gene Symbol: Hc
• Uniprot:
  • P06684()