Complement Component C2b Antibody (269716)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 269716
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Component C2b Antibody (269716) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Complement Component C2
  Ala21-Leu752
  Accession # P06681
• Specificity: Detects human Complement Component C2b in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) C1r, rhC1s, rhC3r, rhC5a, rhFactor B, or rhFactor I is observed. In Western blots, this antibody recognizes rhC2 and rhC2B.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: C2
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C2b Antibody (269716)

• ARMD14
• CO2
• Complement Component C2b

Background

C2 is a key protease involved in the classical pathway of complement. It is synthesized as a single-chain and is proteolytically processed into two chains, C2a (aa 244‑752) and C2b (aa 21‑243). C2a consists of a vWF domain and a serine protease domain. C2b contains 3 Sushi (SCR) domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Component C2b Antibody (MAB1936)(1)

Publication using MAB1936

• R Spirig, IK Campbell, S Koernig, CG Chen, BJB Lewis, R Butcher, I Muir, S Taylor, J Chia, D Leong, J Simmonds, P Scotney, P Schmidt, L Fabri, A Hofmann, M Jordi, MO Spycher, S Cattepoel, J Brasseit, C Panousis, T Rowe, DR Branch, A Baz Morell, F Käsermann, AW Zuercher rIgG1 Fc Hexamer Inhibits Antibody-Mediated Autoimmune Disease via Effects on Complement and Fc?Rs J. Immunol., 2018;0(0):. 2018 [PMID: 29531170] (Western Blot, Human)

Bioinformatics

• Gene Symbol: C2
• Uniprot:
  • P06681()