Complement Component C1s Antibody (Hu222) [Unconjugated]

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human Complement Component C1s (Research Grade Sutimlimab Biosimilar) Monoclonal Antibody (Catalog # MAB11489) to Complement Component C1s. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A goat anti-human HRP conjugate was used for detection.

• Reactivity: Hu
• Applications: ELISA
• Clone: Hu222
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated

Complement Component C1s Antibody (Hu222) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody For Research Use Only
• Immunogen: NS0 mouse myeloma cell line transfected with human CD38
  Met1-Ile300
  Accession # P28907
• Specificity: Detects human CD38 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C1s Antibody (Hu222) [Unconjugated]

• basic proline-rich peptide IB-1
• C1 esterase
• C1S
• complement C1s subcomponent
• Complement component 1 subcomponent s
• complement component 1, s subcomponent
• Complement Component C1s
• EC 3.4.21
• EC 3.4.21.42
• FLJ44757

Background

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)⁺ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP⁺, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta  cells (4, 5).

1. Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
2. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
3. Sun, L. et al. (1999) J. Cell Biol. 146:1161.
4. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
5. Kato, I. et al. (1995) J. Biol. Chem. 270:30045.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P28907()