Mouse Complement C5 ELISA Kit (Colorimetric)

ELISA: Mouse Complement C5 ELISA Kit (Colorimetric) [NBP2-70049] - Samples were spiked with high concentrations of Mouse Complement C5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Complement C5 ELISA Kit (Colorimetric) [NBP2-70049] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Mouse Complement C5 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse C5 in samples. No significant cross-reactivity or interference between Mouse C5 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.73%
• Intra-Assay: CV% < 4.77%
• Spike Recovery: 84-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: C5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Complement C5 ELISA Kit (Colorimetric)

• anaphylatoxin C5a analog
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
• C5
• complement C5
• complement component 5
• CPAMD4MGC142298
• FLJ17816
• FLJ17822

Background

C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kDa) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: C5