Complement C4a/b Antibody (JM88-13)

Western Blot: Complement C4a/b Antibody (JM88-13) [NBP2-66995] - Analysis of C4 on human liver tissue lysate using anti-C4 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Complement C4a/b Antibody (JM88-13) [NBP2-66995] - Staining C4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Complement C4a/b Antibody (JM88-13) [NBP2-66995] - Analysis of paraffin-embedded human liver tissue using anti-C4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Complement C4a/b Antibody (JM88-13) [NBP2-66995] - Analysis of paraffin-embedded human liver cancer tissue using anti-C4 antibody. Counter stained with hematoxylin.

Product Discontinued

Complement C4a/b Antibody (JM88-13) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Complement C4a/b aa 1,231-1,280 / 1,744. (SwissProt: P0C0L4 Human)
• Localization: Cell junction, Cell projection, Secreted, Synapse.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: C4A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Complement C4a/b Antibody (JM88-13)

• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
• C4
• complement component 4A (Rodgers blood group)
• RG

Background

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C4A