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Mouse Complement C4 ELISA Kit (Colorimetric)

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Mouse Complement C4 ELISA Kit (Colorimetric) - Standard Curve Reference
Mouse Complement C4 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL

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Mouse Complement C4 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse C4 in samples. No significant cross-reactivity or interference between Mouse C4 and analogues was observed.
Standard Curve Range
1.56 - 100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.26%
Intra-Assay
CV% < 4.73%
Spike Recovery
86-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
C4A

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Substrate Reagent
  2. Stop Solution
  3. Micro ELISA Plate(Dismountable)
  4. HRP Conjugate Diluent
  5. Reference Standard & Sample Diluent
  6. Reference Standard
  7. Plate Sealer
  8. Product Description
  9. Concentrated Biotinylated Detection Ab (100×)
  10. Certificate of Analysis
  11. Concentrated HRP Conjugate (100×)
  12. Concentrated Wash Buffer (25×)
  13. Biotinylated Detection Ab Diluent

Alternate Names for Mouse Complement C4 ELISA Kit (Colorimetric)

  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
  • C4
  • complement component 4A (Rodgers blood group)
  • RG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&amp;D Systems).
Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.
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NBP2-67656
Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2
Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt, Ze
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Human Complement
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Complement
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Complement C4b/d
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Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.
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Western Blot: ARHGAP4 Antibody [NBP1-88527] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunohistochemistry-Paraffin: ARHGAP4 Antibody [NBP1-88527] - Staining of human colon.
Western Blot: ARHGAP4 Antibody [NBP1-88527] - Analysis in human cell line RPMI-8226.
Species: Hu, Mu, Rt
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PFDN4 Antibody
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Western Blot: PFDN4 Antibody [NBP2-93935] - Analysis of extracts of various cell lines, using PFDN4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/Immunofluorescence: PFDN4 Antibody [NBP2-93935] - Analysis of C6 cells using PFDN4 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PFDN4 Antibody [NBP2-93935] - Paraffin-embedded rat liver using PFDN4.
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Complement Factor
B Antibody
NBP1-89985
Western Blot: Complement Factor B Antibody [NBP1-89985] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: Complement Factor B Antibody [NBP1-89985] - Staining of human cell line ASC TERT1 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100&amp;nbsp;pg/mL.
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Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
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Flow Cytometry: Complement C7 Antibody (15D1) [NBP2-80406] - Analysis of Complement C7 was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
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Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody.
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Intracellular analysis of cleaved D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using 0.5 ug of this antibody was used for this test. Green histogram represents isotype control; red represents anti-D4-GDI antibody. Image using the Alexa Fluor 647 format of this antibody.
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Mouse Complement
C4 ELISA Kit (Colorim ...
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Mouse Complement C4 ELISA Kit (Colorimetric)
Species: Mu
Applications: ELISA

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Product General Protocols

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FAQs for Complement C4 ELISA Kit (NBP2-70040). (Showing 1 - 1 of 1 FAQ).

  1. Hello I was interested in NBP2-70040 Mouse Complement C4 ELISA Kit (Colorimetric) and I was wondering if there is any cross reactivity specifically with human
    • Our lab has tested kit NBP2-70040 with human C4 and they found the cross reactivity to be less than 0.5%.

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  Manual - Mouse Complement C4 ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol C4A