Human Complement C4 ELISA Kit (Colorimetric)

ELISA: Human Complement C4 ELISA Kit (Colorimetric) [NBP2-60619] - Representative standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma
• Standard Curve Range: 0.313-5 ug/mL (example only; lot dependent)
• Sensitivity: 0.25 ug/mL (example only; lot dependent)

Human Complement C4 ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.313-5 ug/mL (example only; lot dependent)
• Sensitivity: 0.25 ug/mL (example only; lot dependent)
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 8.70 (example only; lot dependent)
• Intra-Assay: CV% < 4.40 (example only; lot dependent)
• Spike Recovery: 94.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: C4A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Complement C4 ELISA Kit (Colorimetric)

• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
• C4
• complement component 4A (Rodgers blood group)
• RG

Background

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Complement C4 ELISA Kit (NBP2-60619)(1)

Publication using NBP2-60619

• Zuo Y, Navaz S, Tsodikov A et al. Anti-Neutrophil Extracellular Trap Antibodies in Antiphospholipid Antibody-Positive Patients: Results From the Antiphospholipid Syndrome Alliance for Clinical Trials and InternatiOnal Networking Clinical Database and Repository Arthritis & rheumatology (Hoboken, N.J.) 2023-03-02 [PMID: 36862141] (IF/ICC)

Bioinformatics

• Gene Symbol: C4A