Complement C3b Antibody (8G4) [Alexa Fluor® 594]

Product Discontinued

Complement C3b Antibody (8G4) [Alexa Fluor® 594] Summary

• Immunogen: C3 isolated from human plasma adsorbed onto aluminum hydroxide gel
• Specificity: Specific for an allotypic marker on human C3 (Leu instead of Pro in codon 314 of exon 9), which occurs regulsaly in the beta-chain of C3F and occasionally in the beta-chain of C3S (F and S mean fast and slow, respectively, on agarose gel electrophoresis) This antibody reacts with the 75-kDa beta-chain band on SDS-PAGE immunoblotting of reduced C3, and reacts with 20-kDa and 17-kDa beta-chain fragments produced by cyanogen bromide cleavage.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Complement C3b Antibody (8G4) [Alexa Fluor® 594]

• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• complement C3
• complement component 3
• complement component C3
• CPAMD1acylation-stimulating protein cleavage product

Background

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of alpha and beta chains (110 kDa and 75 kDa respectively) held together by disulfide bonds (1,2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C3