Complement C3b Antibody (8G4) - BSA Free

Product Discontinued

Complement C3b Antibody (8G4) - BSA Free Summary

• Immunogen: C3 isolated from human plasma adsorbed onto aluminum hydroxide gel
• Specificity: Specific for an allotypic marker on human C3 (Leu instead of Pro in codon 314 of exon 9), which occurs regulsaly in the beta-chain of C3F and occasionally in the beta-chain of C3S (F and S mean fast and slow, respectively, on agarose gel electrophoresis) This antibody reacts with the 75-kDa beta-chain band on SDS-PAGE immunoblotting of reduced C3, and reacts with 20-kDa and 17-kDa beta-chain fragments produced by cyanogen bromide cleavage.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:8000
  • Western Blot

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement C3b Antibody (8G4) - BSA Free

• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• complement C3
• complement component 3
• complement component C3
• CPAMD1acylation-stimulating protein cleavage product

Background

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of alpha and beta chains (110 kDa and 75 kDa respectively) held together by disulfide bonds (1,2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C3