Common gamma Chain/IL-2 R gamma Antibody (38024) [Unconjugated]

Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑2 (30 ng/mL) is neutralized (green line) by increasing concen­trations of Mouse Anti-Human Common gamma Chain/IL‑2 R gamma Monoclonal Antibody (Catalog # MAB284). The ND50 is typically 2.5-15 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 38024
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Common gamma Chain/IL-2 R gamma Antibody (38024) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Common gamma Chain/IL‑2 R gamma
  Leu23-Asn254
  Accession # P31785
• Specificity: Detects human Common gamma Chain/IL‑2 R gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rhIL‑2 R beta, rhIL‑1 RI, rhIL‑1 RII, rhIL‑4 R, rhIL‑6 R, rhIL‑7 R, or rhIL‑10 R is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RG
• Purity: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 2.5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from ascites
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Common gamma Chain/IL-2 R gamma Antibody (38024) [Unconjugated]

• CD132 antigen
• CD132
• CIDX
• combined immunodeficiency, X-linked
• common cytokine receptor gamma chain
• Common gamma Chain
• common gamma-chain
• cytokine receptor common subunit gamma
• gamma(c)
• IL-2 R gamma
• IL-2 receptor subunit gamma
• IL2R gamma
• IL-2R subunit gamma
• IL2RG
• IL-2RG
• IMD4
• interleukin 2 receptor, gamma
• Interleukin-2 receptor subunit gamma
• P64
• SCIDX
• SCIDX1
• severe combined immunodeficiency

Background

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma  chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma _c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Common gamma Chain/IL-2 R gamma Antibody (MAB284)(2)

Publications using MAB284

• Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients. J. Immunol., 2009;182(12):7389-97. 2009 [PMID: 19494261] (ELISA Development, Human)
• Hirst SJ, Hallsworth MP, Peng Q Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain. Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71. 2002 [PMID: 11956062] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL2RG
• Uniprot:
  • P31785()