Common gamma Chain/IL-2 R gamma Antibody (31134) [Unconjugated]

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 31134
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Common gamma Chain/IL-2 R gamma Antibody (31134) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Common gamma Chain/IL‑2 R gamma
  Leu23-Asn254
  Accession # P31785
• Specificity: Detects human Common gamma Chain/IL‑2 R gamma in direct ELISAs.
• Details of Functionality: ActivityAssaywCitation not found None
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RG
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Common gamma Chain/IL-2 R gamma Antibody (31134) [Unconjugated]

• CD132 antigen
• CD132
• CIDX
• combined immunodeficiency, X-linked
• common cytokine receptor gamma chain
• Common gamma Chain
• common gamma-chain
• cytokine receptor common subunit gamma
• gamma(c)
• IL-2 R gamma
• IL-2 receptor subunit gamma
• IL2R gamma
• IL-2R subunit gamma
• IL2RG
• IL-2RG
• IMD4
• interleukin 2 receptor, gamma
• Interleukin-2 receptor subunit gamma
• p64
• SCIDX
• SCIDX1
• severe combined immunodeficiency

Background

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma  chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma _c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.

1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Noguchi, M. et al. (1993) Science 262:1877.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Common gamma Chain/IL-2 R gamma Antibody (MAB2841)(2)

Publications using MAB2841

• Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. PLoS Pathog., 2012;8(1):e1002486. 2012 [PMID: 22253598] (Flow Cytometry, Human)
• Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops. J. Immunol., 2005;175(6):3656-62. 2005 [PMID: 16148110] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL2RG
• Uniprot:
  • P31785()