Common beta Chain Antibody (CSL311) - Low Endotoxin, Azide and BSA Free

GM-CSF-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 4.105 nM.

Immobilized human CSF2RB His at 2 ug/mL can bind Common beta Chain Antibody (CSL311), EC50=0.0122 ug/mL.

IL-3-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 0.2109 nM.

Human CSF2RB HEK293 cells were stained with Common beta Chain Antibody (CSL311) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.049 ug/mL.

• Reactivity: Hu
• Applications: ELISA, Flow, Func
• Clone: CSL311
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated
• Format: Low Endotoxin, Azide and BSA Free
• Concentration: LYOPH

Common beta Chain Antibody (CSL311) - Low Endotoxin, Azide and BSA Free Summary

• Description: The heavy chain type is huIgG4, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
  
  This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
  
  Patent #: WO2023173173A
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: CSF2Rb / CD131
• Source: CHO
• Isotype: IgG4
• Clonality: Monoclonal
• Host: Human
• Gene: CSF2RB
• Purity: Protein A purified
• Endotoxin Note: < 0.001EU/ug,determined by LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional

Packaging, Storage & Formulations

• Storage: Store at -20C in powder form. Store at -80C once reconstituted.
• Buffer: Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Alternate Names for Common beta Chain Antibody (CSL311) - Low Endotoxin, Azide and BSA Free

• beta, low-affinity
• CD131 antigen
• CD131
• CDw131
• colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage)
• Common beta Chain
• CSF2RB
• cytokine receptor common subunit beta
• GM-CSF R beta
• GM-CSF/IL-3/IL-5 receptor common beta subunit
• GM-CSF/IL-3/IL-5 receptor common beta-chain
• IL-3 R beta
• IL3RB
• IL-5 R beta
• interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity)

Background

Interleukin-3 receptor (IL-3R) is a heterodimer cytokine composed of alpha chain and beta chain. IL-3R beta subunit (CD131, beta C) is a common shared beta chain of the receptor for granulocyte- macrophage colony-stimulating factor (GM-CSF) and IL-5 (1). While the beta chain can not bind to the ligand alone, but it is essential for high affinity ligand binding (2). The cytoplasmic portion of the beta chain contains the major domains necessary for ligand-induced proliferation (3). IL-3R beta is expressed in neutrophil, eosinophil, monocyte, and hematpoietic stem cells. A point mutation leading to defective IL-3R beta expression has been associated to Human pulmonary alveolar proteinosis (PAP), a rare cause of respiratory failure (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF2RB
• Uniprot:
  • P32927()