Collagen VIII alpha 1 Antibody - BSA Free

Western Blot: Collagen VIII alpha 1 Antibody - BSA Free [NBP3-47562] - HepG2 cells were subjected to SDS PAGE followed by western blot with (Collagen VIII alpha 1 antibody) at dilution of 1:500

Immunoprecipitation: Collagen VIII alpha 1 Antibody - BSA Free [NBP3-47562] - IP Result of anti-Collagen VIII alpha 1 (IP:, 4ug; Detection: 1:500) with HepG2 cells lysate 1800ug.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Collagen VIII alpha 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Collagen VIII alpha 1 Antibody - BSA Free (NBP3-47562) is a polyclonal antibody validated for use in WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: collagen, type VIII, alpha 1. (Uniprot# P27658)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL8A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunoprecipitation 1:500-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Collagen VIII alpha 1 Antibody - BSA Free

• alpha-1 polypeptide
• chromosome 3 open reading frame 7
• collagen, type VIII, alpha 1
• smooth muscle cell-expressed and macrophage conditioned medium-induced proteinsmag-64

Background

The Collagen VIII alpha 1 gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and amajor component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either ahomo- or a heterotrimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL8A1