Collagen VII Antibody

Western Blot: Collagen VII Antibody [NBP1-00174] - Analysis of Collagen VII in rat testis lysate (35ug protein in RIPA buffer) using this antibody at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Rt, Hu, Mu, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Collagen VII Antibody Summary

• Immunogen: Peptide with sequence C-TVQYSDDPRTEF corresponding to internal region according to NP_000085.1.
• Specificity: This product does not work on cryostat sections of Normal Human Skin.
• Predicted Species: Human (100%), Mouse (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: COL7A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 1.5 ug/ml
• Application Notes: An anonymous customer has reported unsuccessful usage on cryosections of human Skin. WB: Approx. 250 kDa band observed in rat testis lysates (calculated MW of 159 kDa band according to rat NP_001100328.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen VII Antibody

• Collagen 7
• collagen alpha-1(VII) chain
• collagen VII, alpha-1 polypeptide
• collagen, type VII, alpha 1
• Collagen-7
• EBD1
• EBDCT
• EBR1
• epidermolysis bullosa, dystrophic, dominant and recessive
• LC collagen
• long chain collagen
• Long-chain collagen

Background

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen VII Antibody (NBP1-00174)(2)

Publications using NBP1-00174

• Nystrom A, Bornert O, Kuhl T et al. Impaired lymphoid extracellular matrix impedes antibacterial immunity in epidermolysis bullosa Proc. Natl. Acad. Sci. U.S.A. 2018-01-23 [PMID: 29305555] (WB)
• Ortiz-Urda S, Garcia J, Green CL et al. Type VII collagen is required for Ras-driven human epidermal tumorigenesis. Science 2005-03-18 [PMID: 15774758]

Bioinformatics

• Gene Symbol: COL7A1
• Entrez:
  • 1294(Human)
  • 12836(Mouse)
  • 301012(Rat)
• Uniprot:
  • NP_000085.1(Human)