Collagen IV Antibody (COL-94)

Immunohistochemistry-Paraffin: Collagen IV Antibody (COL-94) [NBP1-22632] - Formalin fixed paraffin embedded human placenta stained with Collagen IV antibody. (NBP1-22632)

• Reactivity: Hu
• Applications: IHC
• Clone: COL-94
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Collagen IV Antibody (COL-94) Summary

• Description: Novus Biologicals Mouse Collagen IV Antibody (COL-94) (NBP1-22632) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human collagen type IV.
• Localization: Basement membrane
• Specificity: This antibody recognizes collagen type IV epitope located on the alpha1 and/or alpha 2 chains of human collagen type IV. This shows no cross reactivity with collagen types I, II, III, V, VI and VII and does not react with human vitronectin, fibronectin or chondroitin sulfate A, B and C.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL4A1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Paraffin
• Application Notes: IHC-P: recommended pretreatment of HistoZyme. Recommended incubation time of 30 min at RT.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Ascites

Alternate Names for Collagen IV Antibody (COL-94)

• BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
• Collagen IV

Background

Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL4A1