Collagen IV alpha 1 Antibody [HRP]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: HRP

Collagen IV alpha 1 Antibody [HRP] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Collagen IV alpha 1
  Ser1441-Thr1669
  Accession # P02462
• Specificity: Detects human Collagen IV alpha 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse COL13A1, recombinant human (rh) COL25A1 and rhCOL23A1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: COL4A1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Collagen IV alpha 1 Antibody [HRP]

• alpha-1 chain
• Arresten
• COL4A1 NC1 domain
• COL4A1
• collagen alpha-1(IV) chain
• Collagen IV alpha 1
• collagen IV, alpha-1 polypeptide
• collagen, type IV, alpha 1
• Collagen-4
• EC 3.4.23
• EC 6.3.1.2

Background

Collagen IV is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL4A1