Collagen II Antibody

Product Discontinued

Collagen II Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 101-150 of Human COL2A1.
• Specificity: Collagen II (P133) detects endogenous levels of Collagen II (P133) protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Collagen II (P133) pAb in extracts from COLO205 cell. Immunohistochemistry (IHC) analyzes of Collagen II (P133) pAb in paraffin-embedded human breast carcinoma tissue. Use in IHC-frozen sections was reported in scientific literature (PMID: 23762363).
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Collagen II Antibody

• Alpha-1 type II collagen
• ANFH
• AOM
• cartilage collagen
• Chondrocalcin
• COL11A3
• COL2A1
• Collagen 2
• collagen alpha-1(II) chain
• Collagen II
• collagen II, alpha-1 polypeptide
• collagen type 2
• collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
• collagen, type II, alpha 1
• Collagen-2
• MGC131516
• SEDC

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen II Antibody (NB100-91715)(3)

Publications using NB100-91715

• Kim H, Choi YJ, Lee YS et al. SLIT3 regulates endochondral ossification by b-catenin suppression in chondrocytes. Biochem. Biophys. Res. Commun. 2018-12-02 [PMID: 30389141] (WB, Mouse)
• Cheng A, Cain SA, Tian P et al. Recombinant Extracellular Matrix Protein fragments Support Human Embryonic Stem Cell Chondrogenesis. Tissue Eng Part A 2017-12-27 [PMID: 29279011] (Human)
• Liu CF, Breidenbach A, Aschbacher-Smith L et al. A Role for Hedgehog Signaling in the Differentiation of the Insertion Site of the Patellar Tendon in the Mouse. PLoS One 2013-06-10 [PMID: 23762363] (IHC-Fr, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: COL2A1
• Entrez:
  • 1280(Human)
  • 25412(Rat)
• Uniprot:
  • P02458(Human)