COL14A1 Antibody

Western Blot: COL14A1 Antibody [NBP2-15940] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:500.

Immunohistochemistry-Frozen: COL14A1 Antibody [NBP2-15940] - Jaculus jaculus metatarsals with skin. Green: Pro-collagen 1 in osteoblasts, Blue: DAPI, Red: COL14 in periosteum and skin. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: COL14A1 Antibody [NBP2-15940] - Human breast cancer, using COL14A1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Discontinued

COL14A1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human COL14A1. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Predicted Species: Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL14A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: COL14A1 antibody validated for IHC-F from a verified customer review.
• Theoretical MW: 194 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%). Customer validated species reactivity: Jaculus jaculus.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for COL14A1 Antibody

• collagen alpha-1(XIV) chain
• collagen, type XIV, alpha 1
• undulin (fibronectin-tenascin-related)
• Undulin
• UNDundulin

Background

Type XIV collagen is a fibril-associated collagen with an interrupted triple helix. It interacts with the fibril surface and regulates fibrillogenesis (Ansorge et al., 2009 [PubMed 19136672]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for COL14A1 Antibody (NBP2-15940)(3)

Publications using NBP2-15940

• Wang J, Rattner A, Nathans J Bacterial meningitis in the early postnatal mouse studied at single-cell resolution eLife 2023-06-15 [PMID: 37318981] (IHC, Mouse)
• Ishibashi K, Ikegami K, Shimbo T et al. Single-cell transcriptome analysis reveals cellular heterogeneity in mouse intra- and extra articular ligaments Communications biology 2022-11-12 [PMID: 36371589] (IHC-Fr, Mouse)
• Tavallaee G, Lively S, Rockel JS et al. Contribution of microRNA-27b-3p to synovial fibrotic responses in knee osteoarthritis Arthritis & rheumatology (Hoboken, N.J.) 2022-07-06 [PMID: 35791923] (IF/IHC, Mouse)

Review for COL14A1 Antibody (NBP2-15940) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Jaculus Jaculus.

reviewed by: Verified Customer IHC-Fr Jaculus Jaculus 06/28/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Metatarsals with skin
• Species: Jaculus Jaculus
• Lot: 40289

Bioinformatics

• Gene Symbol: COL14A1
• Uniprot:
  • Q05707(Human)