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COL14A1 Antibody

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Western Blot: COL14A1 Antibody [NBP2-15940] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:500.
Western Blot: COL14A1 Antibody [NBP2-15940] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Frozen: COL14A1 Antibody [NBP2-15940] - Jaculus jaculus metatarsals with skin. Green: Pro-collagen 1 in osteoblasts, Blue: DAPI, Red: COL14 in periosteum and skin. Image submitted by a verified ...read more
Immunohistochemistry-Paraffin: COL14A1 Antibody [NBP2-15940] - Human breast cancer, using COL14A1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-15940
    • Availability
      Product Discontinued

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COL14A1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human COL14A1. The exact sequence is proprietary.
Localization
Secreted; extracellular space; extracellular matrix
Predicted Species
Mouse (92%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL14A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Application Notes
COL14A1 antibody validated for IHC-F from a verified customer review.
Theoretical MW
194 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-15940 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%). Customer validated species reactivity: Jaculus jaculus.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for COL14A1 Antibody

  • collagen alpha-1(XIV) chain
  • collagen, type XIV, alpha 1
  • undulin (fibronectin-tenascin-related)
  • Undulin
  • UNDundulin

Background

Type XIV collagen is a fibril-associated collagen with an interrupted triple helix. It interacts with the fibril surface and regulates fibrillogenesis (Ansorge et al., 2009 [PubMed 19136672]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).
Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586] - Canine brain/spinal cord. VWF antibody in green. A pericyte marker in red. Alexa Fluor conjugated secondary antibodies were used for visualization. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.
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(6 Publications)
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Immunohistochemistry-Paraffin: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of paraffin-embedded mouse kidney tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
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Immunohistochemistry-Paraffin: p97/VCP Antibody (5) [NB120-11433] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
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Immunohistochemistry: LOX Antibody [NB100-2527] - IR-enhanced LOX in tumor xenografts. Tumor tissue from A549-derived tumor xenografts treated with 1 x 10 Gy and 2 x 10 Gy (with 12 h between fractions) and evaluated by immunohistochemistry for LOX at different time points after irradiation. Whole tumor sections were quantified for specific LOX-staining intensity. Each treatment group consists of 3 animals. At least 3 sections per tumor/animal were analyzed. Image collected and cropped by CiteAb from the following publication (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human fallopian tube shows moderate cytoplasmic postivity in glandular cells.
Immunohistochemistry-Paraffin: PLEKHM1 Antibody [NBP2-38449] - Staining of human placenta shows moderate granular cytoplasmic postivity in trophoblastic cells.
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Immunohistochemistry-Frozen: CD11b Antibody [NB110-89474] - Immunohistochemical analysis of frozen sections of mouse spleen using [NB110-89474]. IHC-Fr image submitted by a verified customer review.
Flow Cytometry: CD11b Antibody [NB110-89474] - A surface stain was performed on Raw264.7 cells with DyLight 550-conjugated [NB110-89474R] (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Review for COL14A1 Antibody (NBP2-15940) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Jaculus Jaculus.

Reviews using NBP2-15940:
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Immunohistochemistry-Frozen COL14A1 NBP2-15940
Enlarge 		5
reviewed by:
L P
 Immunohistochemistry-Frozen Jaculus Jaculus 06/28/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMetatarsals with skin
SpeciesJaculus Jaculus
Lot40289

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Immunohistochemistry-Frozen COL14A1 NBP2-15940
Enlarge
 L P
06/28/2018
Application: Immunohistochemistry-Frozen
Species: Jaculus Jaculus
read this review

Bioinformatics

Gene Symbol COL14A1
Uniprot