COBRA1 Antibody

Images

 
Western Blot: COBRA1 Antibody [NBP2-15935] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: COBRA1 Antibody [NBP2-15935] - Immunofluorescence analysis of methanol-fixed H1299, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP2-15935] - Antibody detects COBRA1 protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. COBRA1 antibody ...read more
Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP2-15935] - Nucleus on gastric carcinoma; antibody dilution: 1:500.
Immunohistochemistry-Paraffin: COBRA1 Antibody [NBP2-15935] - COBRA1 antibody detects COBRA1 protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. COBRA1 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

COBRA1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human COBRA1. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COBRA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (85%), Zebrafish (86%), Xenopus tropicalis (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COBRA1 Antibody

  • cofactor of BRCA1DKFZp586B0519
  • KIAA1182negative elongation factor B
  • NELFB
  • NELF-Bnegative elongation factor protein B

Background

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for COBRA1 Antibody (NBP2-15935)

Discover related pathways, diseases and genes to COBRA1 Antibody (NBP2-15935). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for COBRA1 Antibody (NBP2-15935)

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Blogs on COBRA1.

COBRA1: A Key Player in Transcriptional Pausing
Co-factor of BRCA1, also known as COBRA1, was first identified as a protein that binds to the tumour suppressor protein encoded by the breast cancer susceptibility gene BRCA1 (1). It was subsequently found to be identical to subunit B of the Negative...  Read full blog post.

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Bioinformatics

Gene Symbol COBRA1
Uniprot