WHSC2 Antibody (6B11H8)

Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (5), SPC-A-1 (6) cell lysate, and Rat brain (4) tissue lysate.

Immunocytochemistry/Immunofluorescence: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunofluorescence analysis of HeLa cells using WHSC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WHSC2 mouse mAb with DAB staining.

Flow Cytometry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Flow cytometric analysis of HEK293 cells using WHSC2 mouse mAb (green) and negative control (purple).

Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mAb against human WHSC2 recombinant protein. (Expected MW is 50.2 kDa)

Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mAb against HEK293 (1) and WHSC2 (AA: 280-511)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WHSC2 mouse mAb with DAB staining.

ELISA: WHSC2 Antibody (6B11H8) [NBP2-37394] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
• Clone: 6B11H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

WHSC2 Antibody (6B11H8) Summary

• Immunogen: Purified recombinant fragment of human WHSC2 (AA: 280-511) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: WHSC2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 57.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for WHSC2 Antibody (6B11H8)

• FLJ10442
• negative elongation factor A
• NELFA
• NELF-AFLJ25112
• Wolf-Hirschhorn syndrome candidate 2 protein
• Wolf-Hirschhorn syndrome candidate 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WHSC2