Coagulation Factor VII Antibody (F7/3511)

Immunohistochemistry-Paraffin: Coagulation Factor VII Antibody (F7/3511) [NBP3-07243] - Formalin-fixed, paraffin-embedded human liver stained with Coagulation Factor VII Mouse Monoclonal Antibody (F7/3511).

Protein Array: Coagulation Factor VII Antibody (F7/3511) [NBP3-07243] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Coagulation Factor VII Mouse Monoclonal Antibody (F7/3511).

• Reactivity: Hu
• Applications: ELISA, IHC, MA
• Clone: F7/3511
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Coagulation Factor VII Antibody (F7/3511) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08251)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (around aa 366-466) of human Coagulation Factor VII protein (Uniprot: P08709)
• Localization: Secreted.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: F7
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA).
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Coagulation Factor VII Antibody (F7/3511)

• coagulation factor VII (serum prothrombin conversion accelerator)
• Coagulation Factor VII
• EC 3.4.21
• EC 3.4.21.21
• Eptacog alfa
• F7
• FVII coagulation protein
• proconvertin
• Serum prothrombin conversion accelerator
• SPCA

Background

Factor VII is a 50kDa multi-domain, single chain plasma glycoprotein synthesized in the liver and having abnormal circulating concentration of between 400 and 600 ug/L. It is essential for blood coagulation. Factor VII is converted to the active, two chain, serine protease Factor VIIa through cleavage by thrombin, factor Xa, factor IXa or factor XIIa. Factor VIIa converts factor X to factor Xa, which in turn converts prothrombin to thrombin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F7
• Uniprot:
  • P08709(Human)