Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

• Reactivity: Po
• Applications: ELISA
• Suitable Sample Type: Plasma
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.054 ng/mL (example only; lot dependent)

Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.054 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Spike Recovery: 79 - 94% (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: F3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Porcine Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Coagulation Factor III/Tissue Factor ELISA Kit (NBP3-42890). (Showing 1 - 2 of 2 FAQ).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: F3