Human Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

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Order Details


    • Catalog Number
      KA0506
    • Availability
      Product Discontinued

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Human Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric) Summary

Description
Tissue Factor (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human Tissue Factor.
Standard Curve Range
12.5 to 400 pg/mL
Sensitivity
3.3 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
F3

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes
Useful for quantification.
Publications
Read Publications using KA0506.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Human Tissue Factor Antibody (50x): A 50-fold concentrated biotinylatedpolyclonal antibody against human TF
  2. Chromogen Substrate (1x): A stabilized peroxidase chromogen substratetetramethylbenzidine
  3. Human Tissue Factor Microplate: A 96-well polystyrene microplate coated with a polyclonalantibody against human TF.
  4. Human Tissue Factor Standard: Human TF in a buffered protein base, lyophilized.
  5. MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base
  6. Sealing Tapes: Pressure sensitive sealing tapes that can be cut to fit the format of theindividual assay.
  7. SP Conjugate (100x): A 100-fold concentrate.
  8. Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogen substratereaction.
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Coagulation Factor III/Tissue Factor ELISA Kit (Colorimetric)

  • CD142 antigen
  • CD142
  • coagulation factor III (thromboplastin, tissue factor)
  • Coagulation Factor III
  • F3
  • FLJ17960
  • TF
  • TFA
  • Thromboplastin
  • Tissue Factor

Background

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Coagulation Factor III/Tissue Factor ELISA Kit (KA0506)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Coagulation Factor III/Tissue Factor ELISA Kit (KA0506). (Showing 1 - 2 of 2 FAQs).

  1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
    • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.
  2. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol F3