Coagulation Factor III/Tissue Factor Antibody

Western Blot: Coagulation Factor III/Tissue Factor Antibody [H00002152-B01P] - Analysis of F3 expression in human pancreas.

Flow Cytometry: Coagulation Factor III/Tissue Factor Antibody [H00002152-B01P] - Analysis of negative control 293 cells (Black) and F3 expressing 293 cells (Green) using F3 purified mouse polyclonal antibody.

Western Blot: Coagulation Factor III/Tissue Factor Antibody [H00002152-B01P] - Analysis of F3 expression in A-431.

Western Blot: Coagulation Factor III/Tissue Factor Antibody [H00002152-B01P] - Analysis of F3 expression in transfected 293T cell line by F3 polyclonal antibody. Lane 1: F3 transfected lysate(32.45 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Coagulation Factor III/Tissue Factor Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: F3 (AAH11029.1, 1 a.a. - 295 a.a.) full-length human protein. METPAWPRVPRPETAVARTLLLGWVFAQVAGASGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFREIFYIIGAVVFVVIILVIILAISLHKCRKAGVGQSWKENSPLNVS
• Specificity: F3 - coagulation factor III (thromboplastin, tissue factor),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: F3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for FACS.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor III/Tissue Factor Antibody (H00002152-B01P)(1)

Publication using H00002152-B01P

• Sommerville L. J, Gorman K. L, et al. A unique protein kinase C-dependent pathway for tissue factor downregulation in pericytes. J Thromb Haemost 2019-04-01 [PMID: 30698330]

FAQs for Coagulation Factor III/Tissue Factor Antibody (H00002152-B01P). (Showing 1 - 1 of 1 FAQ).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3
• Entrez:
  • 2152(Human)
• Uniprot:
  • AAH11029.1(Human)