Coagulation Factor III/Tissue Factor Antibody (4G4)

Western Blot: Coagulation Factor III/Tissue Factor Antibody (4G4) [H00002152-M01] - F3 monoclonal antibody (M01A), clone 4G4. Analysis of F3 expression in Jurkat.

Western Blot: Coagulation Factor III/Tissue Factor Antibody (4G4) [H00002152-M01] - F3 monoclonal antibody (M01), clone 4G4 Analysis of F3 expression in A-431.

Western Blot: Coagulation Factor III/Tissue Factor Antibody (4G4) [H00002152-M01] - Analysis of F3 expression in transfected 293T cell line by F3 monoclonal antibody (M01A), clone 4G4.Lane 1: F3 transfected lysate(33.1 KDa).Lane 2: Non-transfected lysate.

Western Blot: Coagulation Factor III/Tissue Factor Antibody (4G4) [H00002152-M01] - F3 monoclonal antibody (M01A), clone 4G4 Analysis of F3 expression in A-431.

Proximity Ligation Assay: Coagulation Factor III/Tissue Factor Antibody (4G4) [H00002152-M01] - Analysis of protein-protein interactions between F7 and F3. HeLa cells were stained with anti-F7 rabbit purified polyclonal 1:1200 and anti-F3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Immunoprecipitation: Coagulation Factor III/Tissue Factor Antibody (4G4) [H00002152-M01] - Analysis of F3 transfected lysate using anti-F3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F3 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: Coagulation Factor III/Tissue Factor Antibody (4G4) [H00002152-M01] - Detection limit for recombinant GST tagged F3 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

Coagulation Factor III/Tissue Factor Antibody (4G4) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: F3 (AAH11029, 45 a.a. ~ 154 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTK
• Specificity: F3 - coagulation factor III (thromboplastin, tissue factor)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: F3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody (4G4)

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Coagulation Factor III/Tissue Factor Antibody (H00002152-M01). (Showing 1 - 1 of 1 FAQ).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3
• Entrez:
  • 2152(Human)
• OMIM:
  • 134390(Human)
• Uniprot:
  • AAH11029(Human)