Coagulation Factor III/Tissue Factor Antibody (323519) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor APC-conjugated Monoclonal Antibody (Catalog # FAB23391A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 323519
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Coagulation Factor III/Tissue Factor Antibody (323519) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor
  Gly34-Glu251
  Accession # P13726
• Specificity: Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: F3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor III/Tissue Factor Antibody (323519) [Allophycocyanin]

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).

1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
5. Morrissey, J.H. et al. (1987) Cell 50:129.
6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Coagulation Factor III/Tissue Factor Antibody (FAB23391A)(1)

Publication using FAB23391A

• KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles J. Biol. Chem., 2016-03-22;291(20):10586-601. 2016-03-22 [PMID: 27006397] (Flow Cytometry, Human)

FAQs for Coagulation Factor III/Tissue Factor Antibody (FAB23391A). (Showing 1 - 1 of 1 FAQ).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

Bioinformatics

• Gene Symbol: F3
• Uniprot:
  • P13726()