Coactosin-like Protein 1/CotL1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

Coactosin-like Protein 1/CotL1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human Coactosin-like Protein 1/COTL1
  Ala2-Glu142
  Accession # Q14019
• Specificity: Detects human Coactosin-like Protein 1/COTL1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coactosin-like Protein 1/CotL1 Antibody [Alexa Fluor® 594]

• CLP
• CLPFLJ43657
• Coactosin like Protein 1
• coactosin-like 1 (Dictyostelium)
• Coactosin-like Protein 1
• coactosin-like protein
• COTL1
• MGC19733

Background

COTL1 (Coactosin-like Protein) is both a cytoplasmic and plasma-appearing 15-16 kDa member of the coactosin subfamily, ADF/Actin Depolymerizing Factor family of actin-binding proteins. It is widely expressed, and found in cell types such as neutrophils, and tissues such as placenta, lung and kidney. Functionally, COTL1 interacts noncovalently with both F-actin and 5-lipoxygenase/5LO. These interactions appear to be mutually exclusive. A COTL1:F-actin interaction leads to actin binding without actin polymerization, while a 5LO:COTL1 interaction has two potential outcomes; first, 5LO sequesters COTL1, leading to a failure of actin binding, and second, COTL1 can serve as a scaffold for 5LO activity, facilitating the production of either 5HPETE or LTA4. Human COTL1 is 142 amino acids (aa) in length. It is principally composed of one ADF-H domain (aa 2-130) that possesses a utilized phosphorylation site at Ser115, and two acetylation sites at Lys102 and Lys126. COTL1 may form noncovalent homodimers and oligomers, but not when complexed to F-actin. There is one potential isoform variant that shows a 106 aa substitution for aa 1-53. Full-length human and mouse COTL1 share 95% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.