CNTFR alpha Antibody (121723) [Unconjugated]

CNTFR alpha was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human CNTFR alpha Monoclonal Antibody (Catalog # MAB303) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma and sarcoplasm of skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 121723
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CNTFR alpha Antibody (121723) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CNTF R alpha
  Gln23-Pro346 (predicted)
  Accession # P26992
• Specificity: Detects human CNTF R alpha in direct ELISAs and Western blots. In Western blots, approximately 100% cross-reactivity with recombinant rat CNTF R alpha is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNTFR
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CNTFR alpha Antibody (121723) [Unconjugated]

• Ciliary neurotrophic factor receptor subunit alpha;Cntfr
• CNTF R alpha
• CNTFR alpha
• CNTFR
• CNTFRa

Background

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding alpha  subunit (CNTF R alpha ), and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding human and rat CNTF R alpha share 94% amino acid (aa) sequence identity. Human CNTF R alpha cDNA encodes a 372 aa  precursor protein with a 22 aa residue signal peptide and four potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha  (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CNTFR alpha Antibody (MAB303)(1)

Publication using MAB303

• Puzar DominkusP, Ferdin J, PlemenitasA et al. Nef is secreted in exosomes from Nef.GFP-expressing and HIV-1-infected human astrocytes J. Neurovirol. 7/31/2017 [PMID: 28762184]

Bioinformatics

• Gene Symbol: CNTFR
• Uniprot:
  • P26992()