CNPase Antibody - BSA Free

Western Blot: CNPase Antibody [NBP3-05551] - Western blot analysis of different tissue lysates using goat pAb to CNPase at a dilution of 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNPase protein.

Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP3-05551] - Immunofluorescent analysis of a section of mouse striatum stained with CNPase Antibody at a dilution of 1:3,000 in green, and costained with rabbit pAb to NF-L at a dilution of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNPase antibody stains myelin sheath and the plasma membranes of oligodendrocytes, the myelin producing cells of the CNS. The NF-L antibody labels axons of neuronal cells enclosed by the myelin.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CNPase Antibody - BSA Free Summary

• Immunogen: Full length human recombinant CNPase expressed in E. coli
• Clonality: Polyclonal
• Host: Goat
• Gene: CNP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:3000
  • Western Blot 1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for CNPase Antibody - BSA Free

• 2', 3' cyclic nucleotide 3' phosphohydrolase
• 2'-3'-cyclic nucleotide 3' phosphodiesterase
• 2'-3'-cyclic-nucleotide 3'-phosphodiesterase
• CNP1
• CNPase
• EC 3.1.4.37

Background

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CNPase Antibody (NBP3-05551)(1)

Publication using NBP3-05551

• Eduardo Martin-Lopez, Bowen Brennan, Tianyang Mao, Natalie Spence, Sarah J. Meller, Kimberly Han, Nawal Yahiaoui, Chelsea Wang, Akiko Iwasaki, Charles A. Greer Inflammatory Response and Defects on Myelin Integrity in the Olfactory System of K18hACE2 Mice Infected with SARS-CoV-2 eNeuro 2024-06-04 [PMID: 38834299]

Bioinformatics

• Gene Symbol: CNP