CNPase Antibody

Western Blot: CNPase Antibody [NB110-93362] - Rat brain lysate showing the specific immunolabeling of the ~ 46k CNP protein.

Immunohistochemistry-Frozen: CNPase Antibody [NB110-93362] - Staining of saline treated mouse cortex cryosections with CNPase antibody (green). Cell nuclei are visualized with DAPI DNA stain (blue).

• Reactivity: Hu, Mu, Rt, Sh
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CNPase Antibody Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Endogenous rabbit 2,3 cyclic nucleotide-3-phospho-diesterase. Accession # P13233
• Marker: Oligodendrocyte Marker
• Specificity: Specific for endogenous levels of the ~46 kDa CNPase protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CNP
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CNPase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for CNPase Antibody

• 2', 3' cyclic nucleotide 3' phosphohydrolase
• 2'-3'-cyclic nucleotide 3' phosphodiesterase
• 2'-3'-cyclic-nucleotide 3'-phosphodiesterase
• CNP1
• CNPase
• EC 3.1.4.37

Background

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CNPase Antibody (NB110-93362). (Showing 1 - 1 of 1 FAQ).

1. Has this antibody been tested in rat paraffin tissue sections for IHC or just for WB?
   • This antibody has been shown to stain rat tissue. We will back rat staining with our 100% guarantee for paraffin embedded sections and you may receive a full refund or replacement if this antibody does not perform as specified on the datasheet.

Bioinformatics

• Gene Symbol: CNP
• Entrez:
  • 1267(Human)
  • 12799(Mouse)
  • 25275(Rat)
• Uniprot:
  • P13233(Rat)