CNPase Antibody (CL2887) - Azide and BSA Free

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue

Analysis in human cerebral cortex and pancreas tissues using NBP3-43808 antibody. Corresponding CNPase RNA-seq data are presented for the same tissues.

Staining of human cerebellum shows strong positivity in oligodendrocytes.

Staining of human cerebral cortex shows strong positivity in oligodendrocytes.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of rat cerebral cortex shows strong immunoreactivity in single oligodendrocytes.

Staining of rat brain shows strong positivity in oligodendrocytes in cerebral cortex.

Staining of mouse brain shows strong positivity in oligodendrocytes in the amygdala.

Staining of mouse brain shows strong positivity in oligodendrocytes in the cerebral cortex.

Staining of RH-30 cells using the Anti-CNPase monoclonal antibody) .

Staining of rat brain shows strong positivity in oligodendrocytes in the hippocampus.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL2887
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CNPase Antibody (CL2887) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P09543, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:10000 - 1:20000
  • Immunohistochemistry-Paraffin 1:10000 - 1:20000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.ICC/IF,Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for CNPase Antibody (CL2887) - Azide and BSA Free

• 2', 3' cyclic nucleotide 3' phosphohydrolase
• 2'-3'-cyclic nucleotide 3' phosphodiesterase
• 2'-3'-cyclic-nucleotide 3'-phosphodiesterase
• CNP1
• CNPase
• EC 3.1.4.37

Background

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNP