CNP/NPPC Antibody (347625) - Propeptide

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 347625
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CNP/NPPC Antibody (347625) - Propeptide Summary

• Immunogen: E. coli-derived recombinant human CNP
  Lys24-Cys126
  Accession # P23582
• Specificity: Detects human CNP Propeptide in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-react with recombinant human (rh) ANP precursor or rhBNP precursor is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: NPPC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CNP/NPPC Antibody (347625) - Propeptide

• CNP
• CNP2
• C-type natriuretic peptide
• natriuretic peptide C
• natriuretic peptide precursor C
• NPPC

Background

CNP belongs to the natriuretic peptide family and functions in an autocrine or paracrine fashion. CNP interacts with the GC-B/NPR-B receptor to promote vasorelaxation, vascular remodeling, and the growth and differentiation of bone and neural tissue. CNP is synthesized as a prohormone that is cleaved intracellularly by furin, yielding a 49 aa propeptide (aa 24‑72) and a 53 aa mature peptide (aa 74‑126). Additional cleavage of the mature peptide generates 29 aa (aa 98‑126) and 22 aa (aa 105‑126) peptides, which have comparable activity in some assays. The human CNP prohormone shares 91% and 94% aa sequence identity with mouse and rat, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPPC
• Uniprot:
  • P23582()