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CLPTM1L Antibody

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Western Blot: CLPTM1L Antibody [NBP2-76385] - Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, MCF-7, Hep-G2, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLPTM1L antibody NBP2-76385 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: CLPTM1L Antibody [NBP2-76385] - Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, MCF-7, Hep-G2, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLPTM1L ...read more
Independent Antibodies: Immunoprecipitation: CLPTM1L Antibody [NBP2-76385] - Detection of human CLPTM1L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity Hu, Pm-OrSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

        

Order Details

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CLPTM1L Antibody Summary

Immunogen
Immunogen sequence between amino acids 150 and 200
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CLPTM1L
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoprecipitation 50-100 ul/mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
Tris-buffered Saline containing 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Antigen Affinity-purified

Alternate Names for CLPTM1L Antibody

  • cisplatin resistance related protein CRR9p
  • Cisplatin resistance-related protein 9
  • cleft lip and palate transmembrane protein 1-like protein
  • CLPTM1-like protein
  • CLPTM1-like
  • CRR9DKFZp761M2324
  • CRR9p
  • DKFZp666M1010
  • FLJ14400
  • FLJ32533

Background

CLPTM1L is a membrane protein whose overexpression in cisplatin-sensitive cells causes apoptosis. Polymorphisms in its coding sequence have been reported to increase susceptibility to several cancers, including lung, pancreatic, and breast cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Human Pancreatic carcinoma showing strong staining of tumor cells.
Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.
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Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.
Western Blot: DAT1 Antibody (mAb16) [NBP2-22164] - Analysis of DAT1 expression in rat striatal membrane tissue lysate.
Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.
(3 Reviews)
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Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.
Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)
Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
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Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment and validation of tagged exocyst subunit cell lines. Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles (EXO70, SEC5, and SEC8), or in one allele (SEC3 and SEC6). A shRNA specific to SEC3 was used to confirm the identity of multiple bands in the blot. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-018-07467-5) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of human tonsil tissue stained with rSec6, mAb (9H5) at 10ug/ml.
Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of pig ESK-4 cell lysate.
(6 Publications)
Species: Bv, Ca, Ch, Ha, Hu, Pm, Mu, Po, Rb, Rt, Sh, Ye
Applications: IB, ICC/IF, IHC, IHC-P, WB
Recombinant Human
AHRR GST (N-Term) Pro ...
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Applications: ELISA, AP, PA, PAGE, WB
Cytochrome P450
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NB100-74398
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescentred phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1/1A2 Antibody (MC1) [NB100-74398] - Analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
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Immunohistochemistry-Paraffin: SLC6A18 Antibody [NBP1-82024] - Staining of human lymph node shows low expression as expected.
Immunohistochemistry-Paraffin: SLC6A18 Antibody [NBP1-82024] - Staining of human kidney shows moderate luminal membranous positivity in renal tubules..
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Western Blot: CLPTM1 Antibody [NBP2-32114] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-CLPTM1 antibody NBP2-32114) used for IP at 6 ug per reaction. CLPTM1 was also immunoprecipitated by rabbit anti-CLPTM1 antibody BL16525. For blotting immunoprecipitated CLPTM1, NBP2-32114was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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BSA Free
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Western Blot: MATN3 Antibody [NBP1-77022] - Rat thymus tissue lysate with MATN3 antibody at (A) 1 and (B) 2 ug/mL.
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Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PR-A and PR-B at approximately 90 and 118 kDa, respectively (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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SLC45A2 Antibody
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Western Blot: SLC45A2 Antibody [NBP1-59786] - Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysate.
Western Blot: SLC45A2 Antibody [NBP1-59786] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.
Western Blot: SLC45A2 Antibody [NBP1-59786] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.
Species: Hu
Applications: WB
Nicotinic
Acetylcholine R alpha ...
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ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow Cytometry: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis of SK-N-SH cells using CHRNA5 mouse mAb (green) and negative control (red).
Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (2D2D10) [NBP2-61677] - Analysis using CHRNA5 mAb against human CHRNA5 (AA: 23-254) recombinant protein. (Expected MW is 52.5 kDa)
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NHE3/SLC9A3 [p
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Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.
Immunohistochemistry: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of verified customer review.
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Western Blot: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 (lot NB100-556-4) used for IP at 6 ug per reaction. HdmX/MDM4 was also immunoprecipitated by a previous lot of this antibody (lot NB100-556-3) and another rabbit anti-HdmX/MDM4 antibody. For blotting immunoprecipitated HdmX/MDM4, NB100-556 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.
(32 Publications)
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Applications: IP, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
(1 Review)
(46 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
BAK Antibody
(SU32-07)
NBP2-67460
Flow Cytometry: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: BAK Antibody (SU32-07) [NBP2-67460] - Analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.
(1 Publication)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
MSH3 Antibody
NBP2-19417
Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human lung cancer. MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human colon carcinoma, using MSH3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: MSH3 Antibody [NBP2-19417] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CLPTM1L Antibody
NBP2-76385
CLPTM1L Antibody
Species: Hu, Pm-Or
Applications: WB, IP

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Bioinformatics

Gene Symbol CLPTM1L