CLPTM1L Antibody - BSA Free

Whole cell lysate (25 µg) from HeLa, RPMI-8226, MCF-7,GaMG, and A-549 cells prepared using NETN lysis buffer.Antibody: Affinity purified rabbit anti-CLPTM1L antibody used for WB at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.

Whole cell lysate (1 mg perIP; 5% of IP loaded) from MCF-7 cells prepared using NETNlysis buffer. Antibodies: Affinity purified rabbit antiCLPTM1L antibody used for IP at 6 µgper reaction. CLPTM1L was also immunoprecipitated by asecond antibody against a different epitope of CLPTM1L(BL23465). For blotting immunoprecipitated CLPTM1L,was used at 0.04 µg/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.

Product Discontinued

CLPTM1L Antibody - BSA Free Summary

• Immunogen: Immunogen sequence between amino acids 150 and 200
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLPTM1L
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 µg/mg lysate
  • Western Blot 1:10,000 - 1:25,000

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Tris-buffered Saline containing 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for CLPTM1L Antibody - BSA Free

• cisplatin resistance related protein CRR9p
• Cisplatin resistance-related protein 9
• cleft lip and palate transmembrane protein 1-like protein
• CLPTM1-like protein
• CLPTM1-like
• CRR9DKFZp761M2324
• CRR9p
• DKFZp666M1010
• FLJ14400
• FLJ32533

Background

CLPTM1L is a membrane protein whose overexpression in cisplatin-sensitive cells causes apoptosis. Polymorphisms in its coding sequence have been reported to increase susceptibility to several cancers, including lung, pancreatic, and breast cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLPTM1L