CLOCK Antibody (8F7)

Western Blot: CLOCK Antibody (8F7) [NBP1-51610] - Western blot analysis using CLOCK mouse mAb against CLOCK-hIgGFc transfected HEK293 cell lysate.

Immunocytochemistry/Immunofluorescence: CLOCK Antibody (8F7) [NBP1-51610] - Immunofluorescence analysis of Hela cells using CLOCK mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 8F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CLOCK Antibody (8F7) Summary

• Description: Novus Biologicals Mouse CLOCK Antibody (8F7) (NBP1-51610) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-CLOCK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human CLOCK expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLOCK
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for CLOCK Antibody (8F7)

• BHLHE8
• circadian locomoter output cycles protein kaput
• Class E basic helix-loop-helix protein 8
• clock (mouse) homolog
• clock homolog (mouse)
• CLOCK
• EC 2.3.1.48
• hCLOCK
• KAT13D
• KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Background

Circadian Locomotor Output Cycles Kaput (CLOCK) is a bHLH/PAS protein that plays an important role in the molecular clock mechanism. Specifically, CLOCK activates the central circadian loop by stimulating expression of Period and Timeless proteins. CLOCK has also been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

CLOCK levels peak during the late night and the early part of the subjective day. Mutations in CLOCK causes defective transcriptional activity and lengthens the circadian period, leading to abnormal circadian behavior.

CLOCK antibodies are useful tools for research on circadian rythmns, sleep biology and hormonal cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLOCK Antibody (NBP1-51610)(1)

Publication using NBP1-51610

• Zhang C, Chen L, Sun L et al. BMAL1 collaborates with CLOCK to directly promote DNA double-strand break repair and tumor chemoresistance Oncogene 2023-02-02 [PMID: 36725890] (Western Blot, Immunocytochemistry/ Immunofluorescence)

Bioinformatics

• Gene Symbol: CLOCK