CLIC4 Antibody

Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: CLIC4 Antibody [NBP1-33474] - Paraformaldehyde-fixed HeLa, using CLIC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.

Western Blot: CLIC4 Antibody [NBP1-33474] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry: CLIC4 Antibody [NBP1-33474] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CLIC4 antibody (NBP1-33474) at 1:300 dilution.

Western Blot: CLIC4 Antibody [NBP1-33474] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CLIC4 antibody (NBP1-33474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Ze, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CLIC4 Antibody Summary

• Description: Novus Biologicals Rabbit CLIC4 Antibody (NBP1-33474) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CLIC4. The exact sequence is proprietary.
• Localization: Cytoplasm, Cytoplasmic vesicle membrane, Single-pass membrane protein, Nucleus, Cell membrane, Single-pass membrane protein, Cytoplasm, Cytoplasmic vesicle membrane, Nucleus, Cell membrane
• Predicted Species: Mouse (98%), Rat (96%), Rhesus Macaque (99%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLIC4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CLIC4 Antibody

• chloride intracellular channel 4 like
• chloride intracellular channel 4
• chloride intracellular channel protein 4
• CLIC4L
• DKFZp566G223
• FLJ38640
• H1
• huH1
• Intracellular chloride ion channel protein p64H1
• MTCLIC
• P64H1

Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CLIC4