cleavage stimulation factor Overexpression Lysate (Native)

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Western Blot: cleavage stimulation factor Overexpression Lysate (Adult Normal) [NBL1-09555] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for cleavage stimulation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

cleavage stimulation factor Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201128 and based on accession number NM_001324. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa (CSTF1), transcript variant 2, mRNA.
Gene
CSTF1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for cleavage stimulation factor

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for cleavage stimulation factor Overexpression Lysate (Native)

  • CF-1 50 kDa subunit
  • Cleavage stimulation factor 50 kDa subunit
  • cleavage stimulation factor subunit 1
  • cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD
  • cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
  • CSTF 50 kDa subunit
  • CstF-50
  • CstFp50

Background

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for cleavage stimulation factor Lysate (NBL1-09555) (0)

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Bioinformatics

Gene Symbol CSTF1