| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92846-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71329 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27772-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26652-50ul | |||
![Western Blot: Claudin-5 Antibody [NBP2-92846] -analysis of various lysates, using CLDN5 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-92846_rabbit-polyclonal-claudin-5-antibody-western-blot-28112023132836..jpg "Western Blot: Claudin-5 Antibody [NBP2-92846] -analysis of various lysates, using CLDN5 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody [NBP2-92846] - Analysis of U2OS cells using Claudin-5 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92846-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Claudin-5 Antibody - Azide and BSA Free [NBP2-92846] - Immunofluorescence analysis of NIH/3T3 cells using Claudin-5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92846_rabbit-polyclonal-claudin-5-antibody-4220251429069.jpg%3Ft=1706682212)
![Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:3200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-immunohistochemistry-paraffin-141120251843567.jpg "<b>Orthogonal Strategies Validation. </b>Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:3200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-immunohistochemistry-paraffin-1411202518382813.jpg)
![<b>Orthogonal Strategies Validation. </b>Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:3200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-immunohistochemistry-paraffin-141120251833714.jpg)
![Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-immunohistochemistry-paraffin-1411202518335917.jpg)
![Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:3200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-immunohistochemistry-paraffin-1411202518404312.jpg)
![Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-immunohistochemistry-paraffin-141120251830214.jpg)
![<b>Orthogonal Strategies Validation. </b>Unboiled various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-western-blot-141120251838177.jpg)
![<b>Orthogonal Strategies Validation. </b>Unboiled mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-western-blot-1411202518412914.jpg)
![Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Green: Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.Red: beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-immunohistochemistry-paraffin-1411202518392024.jpg)
![Claudin-5 antibody [HL3156] detects Claudin-5 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Claudin-5 stained by Claudin-5 antibody [HL3156] (NBP3-48773) diluted at 1:2000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48773_rabbit-claudin-5-mab-hl3156-immunohistochemistry-paraffin-14112025182605.jpg)
![Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-1G4-Immunocytochemistry-NBP2-02259-img0003.jpg "Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. ICC/IF image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (1G4) [NBP2-02259] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Claudin 5.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-1G4-Immunocytochemistry-Immunofluorescence-NBP2-02259-img0002.jpg)
![Western Blot: Claudin-5 Antibody - BSA Free [NBP3-47483] - Mouse kidney were subjected to SDS PAGE followed by western blot with (CLDN5 antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47483_rabbit-claudin-5-pab-bsa-free-284202512593086.jpg "Western Blot: Claudin-5 Antibody - BSA Free [NBP3-47483] - Mouse kidney were subjected to SDS PAGE followed by western blot with (CLDN5 antibody) at dilution of 1:1000")
![Immunohistochemistry: Claudin-5 Antibody - BSA Free [NBP3-47483] - Immunohistochemistry of paraffin-embedded mouse kidney using (Claudin-5 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-47483_rabbit-claudin-5-pab-bsa-free-2842025138785.jpg)
![ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Samples were spiked with high concentrations of Human Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75332_human-claudin-5-elisa-kit-colorimetric-13220241626156.jpg "ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Samples were spiked with high concentrations of Human Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Claudin-5 ELISA Kit (Colorimetric) [NBP2-75332] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75332_human-claudin-5-elisa-kit-colorimetric-4320248582510.jpg)
![SDS-Page: Recombinant Human Claudin-5 Protein [H00007122-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue](http://images.novusbio.com/fullsize/Recombinant-Human-Claudin-5-Protein-SDS-Page-H00007122-Q01-img0002.jpg "SDS-Page: Recombinant Human Claudin-5 Protein [H00007122-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue")
![ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Samples were spiked with high concentrations of Rat Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75333_rat-claudin-5-elisa-kit-colorimetric-13220241620441.jpg "ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Samples were spiked with high concentrations of Rat Claudin-5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Claudin-5 ELISA Kit (Colorimetric) [NBP2-75333] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75333_rat-claudin-5-elisa-kit-colorimetric-432024932617.jpg)
![Western Blot: Claudin-5 Antibody (SR3444) [NBP3-27772] - Western blot analysis of Claudin-5 expression in human fetal brain lysate.](http://images.novusbio.com/fullsize/nbp3-27772_rabbit-claudin-5-mab-sr3444-75202410133449.jpg "Western Blot: Claudin-5 Antibody (SR3444) [NBP3-27772] - Western blot analysis of Claudin-5 expression in human fetal brain lysate.")
![Western Blot: Claudin 5 Overexpression Lysate (Adult Normal) [NBL1-09247] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 5.](http://images.novusbio.com/fullsize/Claudin-5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09247-img0002.jpg "Western Blot: Claudin 5 Overexpression Lysate (Adult Normal) [NBL1-09247] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 5.")
![Immunohistochemistry: Claudin-5 Antibody (13G6) [NBP3-26652] - Image of Claudin-5 Antibody (13G6) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.77% DAB.](http://images.novusbio.com/fullsize/nbp3-26652_rabbit-claudin-5-mab-13g6-27220248453211.jpg "Immunohistochemistry: Claudin-5 Antibody (13G6) [NBP3-26652] - Image of Claudin-5 Antibody (13G6) diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.77% DAB.")
![Sandwich ELISA: Claudin-5 Antibody (3D8) [H00007122-M01] - Detection limit for recombinant GST tagged CLDN5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Claudin-5-Antibody-3D8-Sandwich-ELISA-H00007122-M01-img0002.jpg "Sandwich ELISA: Claudin-5 Antibody (3D8) [H00007122-M01] - Detection limit for recombinant GST tagged CLDN5 is approximately 0.1ng/ml as a capture antibody.")