Claudin-3 Antibody (CL13129) - Azide and BSA Free

Analysis in human colon and placenta tissues using NBP3-44367 antibody. Corresponding Claudin-3 RNA-seq data are presented for the same tissues.

Staining of human colorectal cancer (metastasis to ovary) shows strong membranous positivity in tumor cells.

Staining of human colon shows strong membranous positivity in glandular cells.

Staining of human placenta shows no positivity in trophoblastic cells as expected.

Staining of human prostate shows strong membranous positivity in glandular cells.

Staining of human ovarian cancer (high grade serous carcinoma) shows strong membranous positivity in tumor cells.

Staining of human ovarian cancer (endometrioid carcinoma) shows strong membranous positivity in tumor cells.

Staining of MCF-7 cells using the Anti-Claudin-3 monoclonal antibody) .

Analysis in human cell line MCF-7 and human cell line SK-MEL-30.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL13129
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Claudin-3 Antibody (CL13129) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Claudin-3 Antibody (CL13129) - Azide and BSA Free (NBP3-18563) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of O15551, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Claudin-3 Antibody (CL13129) - Azide and BSA Free

• C7orf1
• claudin 3
• Claudin3
• Claudin-3
• CLDN3
• Clostridium perfringens enterotoxin receptor 2
• CPE-R 2
• CPE-R2
• CPETR2
• CPETR2CPE-receptor 2
• HRVP1
• Rat ventral prostate.1 protein homolog
• RVP1
• ventral prostate.1 protein homolog
• ventral prostate.1-like protein

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN3