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Claudin-3 Antibody (385021) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
385021
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
FAB4620V-100UG
Alexa Fluor 488
FAB4620G-100UG
Alexa Fluor 594
FAB4620T-100UG
Alexa Fluor 647
FAB4620R-100UG
Alexa Fluor 700
FAB4620N-100UG
Alexa Fluor 750
FAB4620S-100UG
Allophycocyanin
FAB4620A
Fluorescein
FAB4620F
Phycoerythrin
FAB4620P
Unconjugated
MAB4620
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB4620
Carrier Free
FAB4620F
Carrier Free
FAB4620P
Carrier Free
FAB4620A
Carrier Free
MAB4620
Carrier Free
MAB4620-SP
Carrier Free
FAB4620V-100UG
Carrier Free
FAB4620T-100UG
Carrier Free
FAB4620S-100UG
Carrier Free
FAB4620R-100UG
Carrier Free
FAB4620N-100UG
Carrier Free
FAB4620G-100UG
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Claudin-3 Antibody (385021) [Alexa Fluor® 350] Summary

Immunogen
HEK293 human embryonic kidney cell line transfected with human Claudin-3
Met1-Val220
Accession # O15551
Specificity
Stains human Claudin-3 transfectants but not irrelevant transfectants.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CLDN3
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-3 Antibody (385021) [Alexa Fluor® 350]

  • C7orf1
  • claudin 3
  • Claudin3
  • Claudin-3
  • CLDN3
  • Clostridium perfringens enterotoxin receptor 2
  • CPE-R 2
  • CPE-R2
  • CPETR2
  • CPETR2CPE-receptor 2
  • HRVP1
  • Rat ventral prostate.1 protein homolog
  • RVP1
  • ventral prostate.1 protein homolog
  • ventral prostate.1-like protein

Background

Claudin-3 is a 23 kDa multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-3 is upregulated by EGF and in inflammation and a variety of epithelial cancers. Its expression is lost in the blood brain barrier during pathological disruptions of that structure. Claudin-3 binding to the Clostridium perfringes exotoxin induces epithelial cell lysis. Human Claudin-3 shares 91% amino acid sequence identity with mouse and rat Claudin-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Claudin-4 Antibody [NBP2-41187] - Western Blot Validation in Human Colon Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: CLAUDIN4, NBP2-41187 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Claudin-4 Antibody [NBP2-41187] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: CLAUDIN4, NBP2-41187 (1 ug/mL), CLAUDIN4 from Company B (1 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Claudin-4 Antibody [NBP2-41187] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: CLAUDIN4, NBP2-41187 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
(1 Publication)
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Claudin-1 Antibody
(1C5-D9)
H00009076-M01
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in A-431.
Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 5 ug/ml.
Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in PRC/PRF/5.
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(6-G3)
NBP2-66784
Western Blot: Claudin-7 Antibody (6-G3) [NBP2-66784] - Analysis on SW480 cell lysate using anti- CLDN7 mouse mAb.
Immunocytochemistry/Immunofluorescence: Claudin-7 Antibody (6-G3) [NBP2-66784] - Staining CLDN7 in SW480 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Claudin-5 Antibody
(OTI1G4)
NBP2-02259
Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. ICC/IF image submitted by a verified customer review.
Flow Cytometry: Claudin-5 Antibody (1G4) [NBP2-02259] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Claudin 5 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/Immunofluorescence: Claudin-5 Antibody (1G4) [NBP2-02259] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Claudin 5.
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Occludin Antibody
NBP1-87402
Western Blot: Occludin Antibody [NBP1-87402] - Analysis in human cell line CACO-2 and human cell line HeLa.
Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: untreated vascular endothelial cells Lane 2: vascular endothelial cells + control siRNA. Primary antibody was diluted 1:1000. Image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Occludin Antibody [NBP1-87402] - Staining of human cell line A-431 shows localization to plasma membrane & cell junctions. Antibody staining shown in green.
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Tight Junction
Protein 1 Antibody
NBP1-85047
Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in human cell line A-431.
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Rat epithelial cells stained positively with Tight Junction Protein 1. Primary antibody at 1:100. Secondary antibody: goat anti-rabbit-Alexa 488 conjugated at 1:500. ICC/IF image submitted by a verified customer review.
(5 Reviews)
(7 Publications)
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KO
Occludin Antibody
NBP1-87402
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Western Blot: Occludin Antibody [NBP1-87402] - Lane 1: untreated vascular endothelial cells Lane 2: vascular endothelial cells + control siRNA. Primary antibody was diluted 1:1000. Image submitted by a verified customer review.
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Carboxypeptidase
E/CPE Antibody [Uncon ...
AF3587
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E-Cadherin
Antibody [Unconjugate
AF748
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E‑Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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EGF [Unconjugated]
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TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Claudin-8 Antibody
NBP1-59157
Western Blot: Claudin-8 Antibody [NBP1-59157] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml
Immunohistochemistry: Claudin-8 Antibody [NBP1-59157] - Human Intestine
Western Blot: Claudin-8 Antibody [NBP1-59157] - Claudin 8 Antibody [NBP1-59157] - HepG2 cell lysate, Antibody Titration: 0.5-1.0ug/ml
(1 Publication)
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Claudin-18 Antibody
NBP2-32002
Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human stomach shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: Claudin-18 Antibody [NBP2-32002] - analysis in human stomach and liver tissues. Corresponding CLDN18 RNA-seq data are presented for the same tissues.
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Claudin-16 Antibody
NBP2-38777
Immunohistochemistry-Paraffin: Claudin-16 Antibody [NBP2-38777] - Staining of human lymph node shows low expression as expected.
Immunohistochemistry-Paraffin: Claudin-16 Antibody [NBP2-38777] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: Claudin-16 Antibody [NBP2-38777] - Staining in human kidney and lymph node tissues using anti-CLDN16 antibody. Corresponding CLDN16 RNA-seq data are presented for the same tissues.
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Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi
(13 Publications)
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Synaptic
glycoprotein SC2 Anti
NBP2-49230
Western Blot: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Analysis in control (vector only transfected HEK293T lysate) and TECR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: Synaptic glycoprotein SC2 Antibody [NBP2-49230] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
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EGFR Antibody
(DH8.3) - EGFRvIII, M ...
NBP2-50599
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
(4 Publications)
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
MUC2 Antibody
(996/1)
NB120-11197
Immunocytochemistry/Immunofluorescence: MUC2 Antibody (996/1) [NB120-11197] - MS-induced intestinal epithelium injury was CRHR1 dependent.Photomicrographs of immunofluorescence of Mucin 2 (Muc2; mucous-forming protein) (F-J) in proximal colon in all experimental groups. Image collected and cropped by CiteAb from the following publication (nature.com/articles/srep46616), licensed under a CC-BY licence.
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Immunocytochemistry/Immunofluorescence: MUC2 Antibody (996/1) [NB120-11197] - MS-induced intestinal epithelium injury was CRHR1 dependent. Photomicrographs of immunofluorescence of Mucin 2 (Muc2; mucous-forming protein) (F-J) in proximal colon in all experimental groups. Image collected and cropped by CiteAb from the following publication (nature.com/articles/srep46616), licensed under a CC-BY licence.
(1 Review)
(4 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
Claudin-3 Antibody
(385021) [Alexa Fluor ...
FAB4620U
Claudin-3 Antibody (385021) [Alexa Fluor® 350]
Species: Hu
Applications: Flow

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FAQs for Claudin-3 Antibody (FAB4620U) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB4620U
Alexa Fluor 405 FAB4620V
Alexa Fluor 488 FAB4620G
Alexa Fluor 594 FAB4620T
Alexa Fluor 647 FAB4620R
Alexa Fluor 700 FAB4620N
Alexa Fluor 750 FAB4620S
Allophycocyanin FAB4620A
Fluorescein FAB4620F
Phycoerythrin FAB4620P
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CLDN3
Uniprot