Claudin-19 Antibody (693202)

Claudin-19 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissuer using Mouse Anti-Human Claudin-19 Monoclonal Antibody (Catalog # MAB6970) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 693202
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Claudin-19 Antibody (693202) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Claudin-19
  Accession # Q8N6F1
• Specificity: Detects human Claudin-19 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN19
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-19 Antibody (693202)

• claudin 19
• Claudin19
• Claudin-19
• CLDN19

Background

Claudin-19 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-19 is expressed by renal tubular epithelial cells, retinal pigment epithelial cells, and Schwann cells. It interacts with Claudin-16 to form cation-selective paracellular channels in the thick ascending loop of the kidney. Mutations of Claudin-19 are associated with renal disorders of magnesium and calcium reabsorption. Human Claudin-19 shares 95% aa sequence identity with mouse and rat Claudin-19. An alternate splice form of human Claudin-19 has a deletion within the C-terminal cytoplasmic region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-19 Antibody (MAB6970)(4)

Publications using MAB6970

• Viheriälä, T;Hongisto, H;Saari, L;Oksanen, M;Ilmarinen, T;Väärämäki, S;Uusitalo, H;Nevalainen, P;Skottman, H; Novel Human Induced Pluripotent Stem Cell-Based Model for Retinal Pigment Epithelial Cells to Reveal Possible Disease Mechanisms for Macular Degeneration in Pseudoxanthoma Elasticum Journal of ophthalmology 2024-09-21 [PMID: 39347541] (Immunocytochemistry, Human)
• Karema-Jokinen, V;Koskela, A;Hytti, M;Hongisto, H;Viheri�l�, T;Liukkonen, M;Torsti, T;Skottman, H;Kauppinen, A;Nymark, S;Kaarniranta, K; Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients The Journal of biological chemistry 2023-05-01 [PMID: 37137441] (ICC, Human)
• T Viheriälä, J Sorvari, TO Ihalainen, A Mörö, P Grönroos, S Schlie-Wol, B Chichkov, H Skottman, S Nymark, T Ilmarinen Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE Scientific Reports, 2021-01-13;11(1):933. 2021-01-13 [PMID: 33441679] (ICC, Human)
• Stanzel B, Liu Z, Somboonthanakij S, Wongsawad W, Brinken R, Eter N, Corneo B, Holz F, Temple S, Stern J, Blenkinsop T Human RPE stem cells grown into polarized RPE monolayers on a polyester matrix are maintained after grafting into rabbit subretinal space. Stem Cell Reports, 2014-01-02;2(1):64-77. 2014-01-02 [PMID: 24511471] (ICC, Human)

Bioinformatics

• Gene Symbol: CLDN19
• Uniprot:
  • Q8N6F1()