CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Claudin-19 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissuer using Mouse Anti-Human Claudin-19 Monoclonal Antibody (Catalog # MAB6970) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6970-SP | |||
![Western Blot: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection against Immunogen (48.95 KDa) .](http://images.novusbio.com/fullsize/Claudin-19-Antibody-2F2-Western-Blot-H00149461-M02-img0004.jpg "Western Blot: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection against Immunogen (48.95 KDa) .")
![ELISA: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection limit for recombinant GST tagged CLDN19 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Claudin-19-Antibody-2F2-ELISA-H00149461-M02-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Claudin-19 Antibody (2F2) [H00149461-M02] - Tight junction proteins & the transepithelial resistance of iPSC-RPE. RPE cells were cultured on laminin-coated Transwell inserts. a Phalloidin labeling demonstrates the cortical arrangement of actin filaments in iPSC-RPE. b–d Immunostaining of the iPSC-RPE cells revealed surface expression of the tight junction proteins ZO-1 (b), occludin (c), & claudin19 (d). e To assess the barrier function of the iPSC-RPE cells, the resistance across monolayers of cells cultured on laminin-coated Transwell inserts was measured at 2-week intervals, following initiation of the cultures. The net TER was determined by subtracting the resistance across a laminin-coated Transwell insert lacking cells, & multiplying by the surface area of the insert (0.33 cm2). The net TER of the iPSC-RPE cells steadily increased between weeks 2 & 8, until it reached a maximal value of just above 200 Ω.cm2. Resistance recordings were made from four individual cultures. e Error bars represent the mean ± the SEM. Scale bars: a–d = 20 μm. ZO-1 zonula occludens-1, TER transepithelial resistance Image collected & cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0652-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00149461-m02_mouse-monoclonal-claudin-19-antibody-2f2-310202415482262.jpg)
![Western Blot: Claudin-19 Antibody [NBP1-59277] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Claudin-19-Antibody-Western-Blot-NBP1-59277-img0003.jpg "Western Blot: Claudin-19 Antibody [NBP1-59277] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Claudin 19 Overexpression Lysate (Adult Normal) [NBP2-05422] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 19.](http://images.novusbio.com/fullsize/Claudin-19-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05422-img0001.jpg "Western Blot: Claudin 19 Overexpression Lysate (Adult Normal) [NBP2-05422] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Claudin 19.")
