Claudin-10b Antibody (394513)

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Claudin-10 b Isoform Monoclonal Antibody (Catalog # MAB4279, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Product Discontinued

Claudin-10b Antibody (394513) Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Claudin‑10 b Isoform
  Met1-Val228
  Accession # P78369
• Specificity: Detects human Claudin‑10b. Stains human Claudin‑10b transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Claudin-10b Antibody (394513)

• Claudin10b
• Claudin-10b
• CLDN10
• CLDN10;CPETRL3;HELIX;OSPL;OSP-L
• CPETRL3
• OSP-L

Background

Claudin-10 is a 228 amino acid multipass membrane protein in the Claudin family of epithelial tight junction proteins. Two splice forms of human Claudin-10 have been described with a divergent N-terminal extracellular loop. Claudin-10b (Swiss-Prot isoform 1) is relatively cation-permeant compared to Claudin-10a and is widely expressed, while Claudin‑10a is restricted to the kidney cortex. Claudin-10 is overexpressed in some carcinomas. Human Claudin‑10b shares 94% aa sequence identity with mouse and rat Claudin-10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLDN10
• Uniprot:
  • P78369(Human)