Claudin-19 Antibody (2F2) - Azide and BSA Free

Western Blot: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection against Immunogen (48.95 KDa) .

Immunocytochemistry/Immunofluorescence: Claudin-19 Antibody (2F2) [H00149461-M02] - Tight junction proteins and the transepithelial resistance of iPSC-RPE. RPE cells were cultured on laminin-coated Transwell inserts. Immunostaining of the iPSC-RPE cells revealed surface expression of the claudin19. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0652-9), licensed under a CC-BY license.

ELISA: Claudin-19 Antibody (2F2) [H00149461-M02] - Detection limit for recombinant GST tagged CLDN19 is 0.1 ng/ml as a capture antibody.

Immunocytochemistry/ Immunofluorescence: Claudin-19 Antibody (2F2) [H00149461-M02] - Tight junction proteins & the transepithelial resistance of iPSC-RPE. RPE cells were cultured on laminin-coated Transwell inserts. a Phalloidin labeling demonstrates the cortical arrangement of actin filaments in iPSC-RPE. b–d Immunostaining of the iPSC-RPE cells revealed surface expression of the tight junction proteins ZO-1 (b), occludin (c), & claudin19 (d). e To assess the barrier function of the iPSC-RPE cells, the resistance across monolayers of cells cultured on laminin-coated Transwell inserts was measured at 2-week intervals, following initiation of the cultures. The net TER was determined by subtracting the resistance across a laminin-coated Transwell insert lacking cells, & multiplying by the surface area of the insert (0.33 cm2). The net TER of the iPSC-RPE cells steadily increased between weeks 2 & 8, until it reached a maximal value of just above 200 Ω.cm2. Resistance recordings were made from four individual cultures. e Error bars represent the mean ± the SEM. Scale bars: a–d = 20 μm. ZO-1 zonula occludens-1, TER transepithelial resistance Image collected & cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0652-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 2F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Claudin-19 Antibody (2F2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Claudin-19 Antibody (2F2) - Azide and BSA Free (H00149461-M02) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Claudin-19 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CLDN19 (AAH30524, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAVLGGSFLCCTCPEPERPNSSPQPYRPGPSAAAREYV
• Specificity: CLDN19 - claudin 19 (2F2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLDN19
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28524846).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Claudin-19 Antibody (2F2) - Azide and BSA Free

• claudin 19
• Claudin19
• Claudin-19
• CLDN19

Background

The product of this gene belongs to the claudin family. It plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Defects in this gene are the cause of hypomagnesemia renal with ocular involvement (HOMGO). HOMGO is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis associated with severe ocular abnormalities such as bilateral chorioretinal scars, macular colobomata, significant myopia and nystagmus. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-19 Antibody (H00149461-M02)(5)

Publications using H00149461-M02

• Álvarez-Barrios A, Álvarez L, Pereiro R et Al. Elemental mass spectrometry to study metallo-transcriptomic changes during the in vitro degeneration of the retinal pigment epithelium Anal Bioanal Chem 2023-07-29 [PMID: 37507467]
• �lvarez-Barrios A, �lvarez L, Artime E et al. Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium Frontiers in Nutrition 2023-04-17 [PMID: 37139441] (Western Blot, Immunocytochemistry/ Immunofluorescence, Human)
• Hazim RA, Karumbayaram S, Jiang M et al. Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2017-10-02 [PMID: 28969679] (ICC/IF, Human)
• Benedicto I, Lehmann GL, Ginsberg M et al. Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors. Nat Commun. 2017-05-19 [PMID: 28524846] (ICC/IF, Mouse)
• Peng S, Adelman RA, Rizzolo LJ. Minimal effects of VEGF and anti-VEGF drugs on the permeability or selectivity of RPE tight junctions Invest Ophthalmol Vis Sci 2010-06-01 [PMID: 20042644] (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: CLDN19
• Entrez:
  • 149461(Human)
• Uniprot:
  • AAH30524(Human)