Claudin-14 Antibody

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Western Blot: Claudin-14 Antibody [NB100-1070] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Claudin-14 Antibody Summary

Immunogen
Peptide with sequence C-SATHSGYRLNDYV corresponding to C-Terminus according to NP_036262.1.
Localization
Cell Membrane
Specificity
Reported variants represent identical protein (NP_036262.1; NP_652763.1; NP_001139551.1; NP_001139550.1 and NP_001139549.1).
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CLDN14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • ELISA 1:4000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
WB: Approx. 23 kDa band observed in human liver lysate (calculated MW of 25.7 kDa band according to NP_652763.1). IHC successful usage on mouse heart. IHC: This antibody has been successfully used in IHC on mouse heart. Use in IHC-P reported in scientific literature (PMID: 21454667).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Claudin-14 Overexpression Lysate
Publications
Read Publications using
NB100-1070 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine. Mouse reactivity reported in scientific literature (PMID: 21454667).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Claudin-14 Antibody

  • claudin 14
  • Claudin14
  • Claudin-14
  • CLDN14
  • DFNB29
  • human CLDN14 gene10claudin-14

Background

CLDN14 is a component of tight junction (TJ) strands. Defects in CLDN14 are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness (DFNB29).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Claudin-14 Antibody (NB100-1070)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CLDN14
Entrez
Uniprot